FACT SHEET: The Truth about PZP

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EXPANDED SUMMARY OF PZP’S ADVERSE EFFECTS, INCLUDING REFERENCES

PZP — The Pesticide

Porcine zona pellucida (PZP aka ZonaStat-H or Native PZP) is an EPA-registered pesticide derived from the ovaries of slaughtered pigs. PZP is approved for use on wild horses “in areas where they have become a nuisance ….” [20]

Some persons argue that, because PZP does not kill the mare, it is not really a “pesticide.” Actually, PZP does kill. As will be documented in this report, its use is associated with stillborn foals. In the long term, PZP will weaken a herd immunologically, which could swiftly lead to its extinction. So, yes, PZP is a real pesticide.

PZP — an Anti-Vaccine

While touted as a “vaccine,” PZP is actually a perversion of what a true vaccine is supposed to be. Instead of preventing disease, PZP causes disease — auto-immune disease. Thus, PZP is an anti-vaccine.

PZP’s Mode of Action as Stated in the Pesticide Registration Is a Disproved Hypothesis

The registrant of PZP advised the Environmental Protection Agency (EPA) that, based on information from the pesticide’s researcher-manufacturer, PZP works by generating antibodies that “block sperm attachment.” This representation of PZP as a sort of chemical condom was not fact but merely an untested hypothesis, postulated three decades ago. The old hypothesis was disproved by subsequent research. PZP’s manufacturer knew, or should have known, this. The manufacturer should also have been informed and up-to-date regarding the side effects and unintended consequences of PZP. Yet, the manufacturer continued to cite the disproved hypothesis and to deny that PZP has any adverse effects. [7 and 13]

PZP’s True Mode-of-Action

So how does PZP really work? PZP tricks the immune system into waging war on the ovaries. In a meta-analysis of ZP-type contraceptives, Kaur & Prabha (2014) reported that the infertility brought on by such products is ” … a consequence of ovarian dystrophy rather than inhibition of sperm-oocyte interaction.” Thus, PZP’s antibodies “work” not by blocking sperm attachment but by destroying the ovaries. Kaur & Prabha further disclosed that ” … histological examination of ovaries of immunized animals revealed the presence of atretic follicles with degenerating oocytes.” [5] [Atretic follicles are ovarian follicles in an undeveloped state due to immaturity, poor nutrition or systemic disease; manifested by prolonged anestrus.]

Kaur & Prabha’s review concluded that PZP’s antibodies induce ovarian dystrophy, oophoritis (inflammation of the ovaries), destruction of oocytes in all growing follicles, and depletion of resting follicles. The manufacturer of PZP as well as Bureau of Land Management (BLM) should have been aware of these and other findings about the pesticide. Yet they ignored or disregarded any information that was contrary to their personally-preferred but obsolete and false description of PZP’s mode-of-action.

PZP Manufacturer’s Own Research Found Markedly Depressed Estrogen Secretion

In a telling study published back in 1992, the manufacturer of Native PZP, along with colleagues, reported that ” … three consecutive years of PZP treatment may interfere with normal ovarian function as shown by markedly depressed oestrogen secretion.” [6] Thus, despite all the hype about PZP being non-hormonal, the manufacturer knew that ZonaStat-H has an adverse hormonal effect, causing significantly-lowered estrogen. Thus, PZP is an endocrine disruptor. [21] The plummeting estrogen-levels may also reflect the ovarian dystrophy and oophoritis now known to be caused by PZP. Despite personally discovering negative hormonal impacts 23 years ago, PZP’s manufacturer continued to cite misinformation regarding the product’s mode-of-action and endocrine-disruptor side-effects.

PZP Causes Ovarian Cysts

In their 2010 meta-analysis, Gray & Cameron cited a number of studies that found ” … alterations to ovarian function, oophoritis, and cyst formation with PZP treatment (Mahi-Brown et al.1988, Sehgal et al. 1989, Rhim et al. 1992, Stoops et al. 2006, Curtis et al. 2007).” [4] These findings support those of Kaur & Prabha while introducing yet another adverse effect: ovarian cysts. Gray & Cameron’s review also noted that increased irritability, aggression, and masculine behavior had been observed in females following PZP-treatment.

PZP → Endocrine Disruptor → Elevated Testosterone → Masculinizing Effects

Recall that PZP has endocrine-disrupting effects that result in lowered estrogen. Per the observed masculine behavior of treated mares, PZP seems to have a testosterone-elevating effect too. A deficit of estrogen alone would not necessarily manifest in the masculinization of treated females, but an excess of testosterone would. So, it appears that PZP disrupts at least two hormones: estrogen — by substantially lowering it — and testosterone — by substantially elevating it. Adverse effect: Unnatural behavior.

PZP → Ovarian Cysts → Elevated Testosterone → Masculinizing Effects

As discussed above, PZP correlates with abnormal masculine behavior on the part of treated females, a side-effect likely due to elevated testosterone. But in addition to the endocrine-disruption caused by PZP, there could be a second way for testosterone levels to become elevated. Recall that PZP causes ovarian cysts. An Internet search on “ovarian cysts and testosterone” yielded results for polycystic ovary syndrome (PCOS) in women. Interestingly, one of the symptoms of PCOS is high testosterone levels. [12 and 22] The connection between ovarian cysts and elevated testosterone suggests that the ovarian cysts caused by PZP could — either alone or in combination with PZP’s endocrine-disruptor effects — lead to high testosterone levels in treated females, as evidenced by their masculinized behavior.

PZP Causes Additional Adverse Effects

Gray & Cameron’s review also disclosed that, when PZP was administered to the females of a herd, males lost body condition while the oft-claimed improvement in female body condition did not hold up. Further, mares remained sexually active beyond the normal breeding season and had more estrus events.

PZP Selects for Weak Immune Function

Gray & Cameron’s analysis raised the possibility of PZP selecting for immuno-compromised individuals. Here’s why. Because PZP stimulates the immune system, it ironically works “best” — sterilizes faster — in mares that have strong immune-function. Such mares respond to the anti-vaccine and produce quantities of PZP antibodies that destroy their ovaries. But, conversely, PZP may not work at all in mares whose immune-function is weak or depressed. Those mares fail to respond to PZP. They keep getting pregnant and producing foals who, like their dam, suffer from weak immune-function. So, the PZP pesticide works against the very horses that Nature has best equipped for survival against disease while favoring and selecting for the immuno-compromised. Thus, a herd being treated with PZP is undergoing selective breeding for weak immunity, which puts the population at risk for disease — and ultimately, for extinction.

PZP Confers Dubious “Benefit” of Increased Longevity

Gray & Cameron also cited a study that found that “… PZP treated feral horse mares lived longer, resulting in a new age class (>25 years) not present before treatment ….” Exceptionally-long life is an ironic effect of PZP treatments. PZP’s manufacturer actually boasts about it, as if the anomaly were a good thing. However, Gray & Cameron questioned the supposed benefit of mares living much longer than their normal life expectancy. Indeed, such mares take up scarce slots within size-restricted populations. The ultra-elderly mares continue to consume resources for many years, but they no longer contribute to the gene-pool. It is detrimental to a population’s genetic viability to carry significant numbers of sterile herd-members way-beyond their normal life-span.

Research on Wildlife Contraceptives Revealed Stillbirths and Auto-Immune Oophoritis from PZP

There was an even earlier, definitive meta-analysis on wildlife contraceptives. Nettles (1997) reviewed 75 studies available at that time on the subject. Among his findings regarding PZP-use across different species, including horses, were: Stillbirths; altered ovarian structure and cyclicity; interference with normal ovarian function; permanent ovarian damage; and some cases of irreversible sterility due to auto-immune oophoritis, which suggested that PZP can be selective against a certain genotype in a population. [10] Many of these findings were confirmed by Kaur & Prabha as well as by Gray & Cameron. Please keep in mind these key findings: Stillbirths, and auto-immune oophoritis.

Recent Stillbirths Correlated with PZP

There is recent evidence confirming Nettles’ finding of a correlation between PZP treatments and subsequent stillbirths. In June 2015, Karen Sussman, President of the International Society for the Protection of Mustangs and Burros, reported that 7 mares previously treated with PZP at ISPMB, when taken off PZP, were able to get pregnant. However, 6 of those 7 mares — that is, 86 percent — produced foals that were stillborn. All other ISPMB mares that had not been injected with PZP successfully birthed healthy foals. Thus, given that environmental and other conditions were identical, the only variable was PZP. The dead foals have been sent to a university pathology department for autopsy. [18]

Autoimmune Oophoritis Induced by PZP

Research by the Rose-Cihakova-Caturegli Laboratory at Johns Hopkins Pathology found: “Automimmune oophoritis can be induced by immunization with testis and ovarian antigen murine human zona pellucida 3 peptide (pZP3) in adjuvant.” [16] Here again, is causation of autoimmune disease by a ZP-type product. This finding confirms other research cited herein.

Autoimmune Oophoritis and Risk of Other Autoimmune Diseases

A study by Varras et al. disclosed that, in humans, autoimmune oophoritis carries the risk of the patient developing other autoimmune diseases. [23] The correlation between autoimmune oophoritis and subsequent other autoimmune disorders weighs against injecting fillies and mares with PZP repeatedly and en masse.

Prolonged Breeding Season, Unusually-late Parturition Dates with PZP

Nettles’ meta-analysis on PZP disclosed other adverse effects: A prolonged breeding season and unusually-late parturition dates. (Parturition is the formal term for “giving birth.”) These findings have recently been confirmed, as is discussed below.

Parturition-Season Extends to Nearly Year-Round When a Herd Is Treated with PZP

A longitudinal study (Ransom et al. 2013) of three herds currently being managed by PZP — Little Book Cliffs, McCullough Peaks, and Pryor Mountain — found that the the parturition season lasted 341 days. [15] Ransom et al.’s finding of a nearly year-round birthing season supports the earlier finding by Nettles. Thus, during its period of potential reversibility, PZP’s effects wear off unpredictably. Out-of-season births put the life of both the mare and the foal in jeopardy. Nature designed the equine birthing-season to occur in Spring, not year-round, and certainly not in the dead of Winter.

Prolonged Delay in Recovery of Fertility

The same longitudinal study by Ransom et al. found that, after suspension of PZP, there was a delay lasting 411.3 days (1.13 years) per each year-of-treatment before mares recovered their fertility. What this means is that it takes that long, on average, for the ovaries to heal, to clear out all those cysts, and to regain some degree of normal hormonal function.

The question is: How is the delay in recovery-of-fertility addressed by BLM management practices? Answer: BLM ignores it. For instance, BLM currently administers PZP to Pryor Mountain’s fillies and mares starting at age 1½ — whom BLM artfully describes in the Environmental Assessment as fillies “becoming two year olds” — through age four. Thus, these fillies and mares receive intentional treatments for four consecutive years before being allowed the privilege of reproductive potentiality. Per Ransom et al.’s study, the Pryor Mountain fillies and mares would be expected to need 1,645.2 days (4.51 years) to regain reproductive capacity. But BLM gives the Pryor Mountain mares only 5 years off PZP before they are put back on it again — for the rest of their life. Thus, these fillies and mares might have just a six-month window — at best — in which to conceive. Due to the unpredictable timing of PZP’s wearing off, for some mares that window of fertility will close before they get a chance to produce a foal. Those mares’ genetic contribution will be zero.

As if the above scenario were not bad enough, PZP’s manufacturer conceded that it could take up to eight years to recover fertility after just three consecutive PZP treatments. [13]

Ransom Advises Proceeding with Caution regarding PZP

The Ransom et al. study warned: “Humans are increasingly attempting to manage the planet’s wildlife and habitats with new tools that are often not fully understood. The transient nature of the immunocontraceptive PZP can manifest into extraordinary persistence of infertility with repeated vaccinations, and ultimately can alter birth phenology in horses. This persistence may be of benefit for managing overabundant wildlife, but also suggests caution for use in small refugia or breeding facilities maintained for repatriation of rare species.” [15]

Because BLM keeps over 70 percent of the herds at levels below minimum-viable population (MVP), most herds qualify as “small refugia.” Pryor Mountain WHR is a small, isolated refuge, and its wild horses carry genes with rare alleles.

Ransom’s Exclusion of Seven Mares Evidences PZP’s Non-Effect on Immunocompromised Mares

In the “Data Collection” methodology section of the Ransom et al. report, the authors advised: “We omitted data for one female from the Little Book Cliffs and six females from McCullough Peaks because they produced offspring in every treatment year and thus were never effectively contracepted.”

This fact is important because it evidences PZP’s lack-of-efficacy on immunocompromised fillies and mares. To review: Because PZP activates the immune system, mares with naturally-low or depressed immune function do not “respond” to the treatment. It’s as if they had been injected with saline — their immune system is so weak that it does not react to the PZP by producing antibodies. The good news is such mares’ ovaries are saved from PZP’s destructive effects. The bad news is that these mares continue to become pregnant year after year, producing foals that will also tend to inherit low immune-function. Over time, the herd will become populated with more such low-immune horses because those with strong immunity get sterilized. Thus, PZP selects for horses with low immune function, which is bad for a herd in the long term. Even a routine infection could spread quickly and wipe out a population of horses with weak immune-function. If the goal is to preserve a herd, the use of PZP constitutes a worst management-practice.

BLM Was Fully-Aware of the Ransom Study but Suppressed the Findings

In their report, the authors of the Ransom et al. study gave a shout-out to BLM “for administrative and technical support throughout this project.” Thus, BLM was fully aware of the multi-year study while it was in progress and even lent support to it administratively and technically. Yet, in the case of the Pryor Mountain herd, BLM omitted this important report as a reference for the 2015 Environmental Assessment, which proposed intensifying the PZP “prescription.” Thus, BLM pretended that there was no such report and unethicallly suppressed it. Consequently, the public could not comment knowledgeably and appropriately on the continued use of — let alone the accelerated application of — PZP.

Three PZP Injections Can Trigger Sterility in Mares, or Just One Shot in Fillies Before Puberty

Disturbingly, another recent study on PZP (Knight & Rubenstein, 2014) found that ” … three or more consecutive years of treatment or administration of the first dose before sexual maturity may have triggered infertility in some mares. [9]

These findings are particularly troubling. They suggest that, actually, only two consecutive PZP-treatments may be reversible. Except, that is, in the case of fillies who have not yet reached puberty — they could be sterilized by just one injection. Recall the Pryor Mountain fillies, whose PZP treatments begin when they are just 1½ years old. They may not have reached puberty when they are initially treated. [1] And as we shall see later in this report, that first shot of PZP may not be their first shot of PZP.

Researchers Again Express Concerns about the Abnormal Life-Spans of Sterilized Mares

Knight & Rubenstein warned: “Inducing sterility, while relieving the mares from the energetic costs of lactation and reducing the stress from harem switching, may have unintended consequences on population dynamics by increasing longevity and eliminating the mares’ ability to contribute genetically.”

Knight & Rubenstein’s concerns support those of Gray & Cameron, who also questioned the supposed benefit of sterile mares’ extended life-spans. The abnormal numbers of aged, sterile mares count for census-purposes; but their presence disadvantages the younger horses, who become tageted for removal in order for BLM to achieve arbitrary management levels. Further, such mares no longer belong to the viable gene-pool.

PZP’s Destructive Antibodies Are Transmitted via the Placenta and Mother’s Milk

It gets worse. Sacco et al. reported that, per radioimmunoassay, PZP antibodies are transferred from mother to young via the placenta and milk. The transferred antibodies cross-react with and bind to the zonae pellucidae of female offspring, as demonstrated by immunofluorescent techniques. [17] These findings were disclosed in 1981. PZP’s manufacturer must have known about this dangerous effect, and certainly BLM should have investigated on its own whether there was any risk to the unborn or the nursing foal. Yet, the manufacturer continued to insist that there was no danger to the foal, whether born or unborn. [7 and 13] And in fact, BLM regularly administers PZP to pregnant and lactating mares, who transfer the destructive antibodies to their fetus, via the placenta, and to their foal, via mother’s milk.

Recall again the Pryor Mountain fillies. If their dams were injected with PZP while pregnant or nursing, such fillies will already have PZP antibodies cross-reacted with and bound to their zonae. Therefore, when those same fillies are injected at age 1½, it will be their second treatment, or potentially even their third. In fact, they could already have been sterilized in utero or while nursing, the treatment having been received prior to puberty, about which Knight & Rubenstein warned.

PZP Weakens Herd-Immunity, Posing Risk of Stochastic Events Leading to Herd-Extinction

To be self-sustaining, a herd needs to possess good immunity to withstand random catastrophes — known as stochastic events — such as contagious infections. There was such an event recently in Kazakhstan, where 120,000 endangered Saiga antelope — half the world’s population — died off suddenly and inexplicably within a two-week period. Scientists think a common bacterial infection was the cause of this mass-mortality event, but are unsure why the antelope were unable to fight off the disease immunologically. [14]

Imagine if such a catastrophe were to befall the Pryor Mountain horses, whose herd-immunity is being eroded by PZP. Note that the Saiga deaths involved antelope-mothers and their calves. If Pryor Mountain’s few fertile mares and their foals perished all of a sudden, that would leave just stallions and sterile old mares. The herd would be composed of the living dead, reproductively speaking, its rare alleles extinguished. BLM is failing to proactively manage the Pryor Mountain herd with stochastic events taken into consideration. That is malfeasance. PZP is a tool of immunological destruction, not of proper management.

PZP Continues the Use of Roundups and Removals

If the promise of PZP were true — if PZP really did eliminate the need to remove “excess” wild horses from the range — removals would have ended long ago in the Pryor Mountain Wild Horse Range, where PZP has been in use for approximately two decades. Yet removals are scheduled there with regularity every three years, the latest one in 2015.

Risks to Humans Who Administer PZP Injections

For staff and volunteers who inject wild horses with PZP, EPA’s Pesticide Fact Sheet advises that Personal Protective Equipment requirements include long sleeved shirt and long pants, gloves and shoes plus socks to mitigate occupational exposure. EPA specifically warns that pregnant women must not be involved in handling or injecting ZonaStat-H, and that all women should be aware that accidental self-injection may cause infertility. [20]

However, EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to inform pregnant women why it is so important that they strictly avoid PZP — because PZP’s antibodies cross the placenta and cross-react with and bind to an unborn female child’s own little zonae pellucidae. The baby-girl could be “anti-vaccinated” with PZP and even sterilized before birth.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to warn lactating women to avoid PZP and why — because PZP’s destructive antibodies would be passed along to a nursing female child via mother’s milk. The baby-girl could be “anti-vaccinated” with PZP and possibly sterilized simply from nursing.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to warn all women of the risk of ovarian dystrophy, oophoritis, ovarian cysts, and elevated testosterone-levels — in addition to infertility and, potentially, sterility — from unintentional self-injection.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to emphasize the magnitude of the risk — the PZP-in-question is a horse-size dose.

But Is There a Mandate to Practice Scientific Integrity?

Yes. The Department of the Interior’s (DOI) Code of Scientific and Scholarly Conduct applies to all staff members as well as to contractors, partners, permittees, and volunteers. The Code states: “Scholarly information considered in Departmental decision making must be robust, of the highest quality, and the result of as rigorous scientific and scholarly processes as can be achieved. Most importantly, it must be trustworthy.” [19]

BLM has ignored and suppressed independent scientific findings about PZP’s adverse effects and unintended consequences. Instead, BLM continues to rely almost exclusively on the manufacturer’s claims — shown and known to be false — regarding PZP’s safety for use on horses and for handling by humans. BLM is thus non-compliant with the Policy and malfeasant in its responsibilities to protect staff, volunteers, and the wild horses under its jurisdiction. BLM is also misleading and disinforming Congress and the American public about the PZP pesticide.

The manufacturer of PZP — a partner to BLM — misrepresented the pesticide as safe for use on animals by humans. The manufacturer knew or should have known that the former hypothesis regarding PZP’s mode-of-action had been disproved, and that PZP has dangerous side effects, safety-issues, and unintended consequences. Yet he hid and denied that information and failed to warn about PZP’s adverse effects. The manufacturer cited his own research as if it were definitive, and aggressively criticized independent researchers whose findings did not fully support his claims. Indeed, he recently submitted an Op-ed to The Salt Lake Tribune wherein he belittled the research of fellow scientists whose studies on PZP yielded results somewhat different from his own. [8] His accusations were so unreasonable that the scientists felt it necessary to submit an Op-ed in response to defend the integrity and validity of their work. [11] The manufacturer also disparaged members of the public — one of whom was a member of the Pennsylvania Game Commission — who expressed concerns about PZP. He dismissively accused them of “an attempt to mislead,” of “hyperbole,” of “knowingly manipulating information,” of “attempts to frighten people,” and of indulging in an “anti-intellectual approach to debates.” [7] By these actions, the manufacturer violated the DOI’s Code of Scientific and Scholarly Conduct.

PZP Manufacturer Misled Trainees into Believing that PZP Was Safe

BLM staff and volunteers receive their training from PZP’s manufacturer in how to handle and administer the pesticide. BLM is remiss in delegating the training to the manufacturer without verifying the adequacy of the instruction and the truthfulness of it. Two comments recorded recently in the media suggest that PZP’s manufacturer misled not just the public-at-large but those who received training therefrom in how to administer PZP.

First, the manufacturer has been quoted as saying that PZP is “so safe it is boring.” [3] Independent research shows otherwise — that PZP is a powerful hormone disruptor that could sterilize a female with just one injection. If trainees believe that PZP is boringly safe, they will be less likely to protect themselves adequately from this dangerous pesticide. Indeed, many of the trainees are women and, therefore, particularly at risk. Likewise, wild-horse advocates are lulled into complacency, trusting that PZP is harmless to the Pryor Mountain horses and their rare genetic alleles. Of course, none of that is true.

Second, a PZP supporter, who self-identified as a recent completer of the PZP-darting training program conducted by the manufacturer, said in a comment posted to a news article: “I just received my FDA certification to handle and administer Native PZP. Would you be so kind to provide a link to the study you keep referencing? To my knowledge, and those teaching the Native PZP certification class, there are no side effects of the PZP produced by Dr. Kirkpatrick and his team, which is Native PZP.” [2] Key words: “no side effects.” It is disturbing that a person who was, no doubt, motivated by a desire to help the horses has been disinformed regarding PZP’s safety-hazards to humans as well as to horses.

BLM Fails to Maintain Proper Supervision of the PZP Volunteers

The issue of safety is not the only concern. As BLM has admitted, volunteers darted the wrong mares on Pryor Mountain. These errors evidence that BLM has failed to maintain supervisory control over the volunteer-inoculators, allowing them to conduct the PZP-darting by themselves. The mistakes further evidence that the volunteers do not understand what is expected of them. Who can say whether other procedures were not complied with either. The fact that mares were darted who were ineligible for PZP per the then-current protocol, but who would be eligible under the proposed-but-not-yet-promulgated new “prescription,” suggests that the volunteers may have concluded — from BLM’s open contempt for the Constitution and disrespect for the NEPA process — that was okay for them to start darting otherwise-ineligible mares right away. Not surprisingly, BLM blames the volunteers for these mistakes, but probably has not informed them that they are being made to take the rap for management’s shortcomings.

Conclusions

PZP is appropriately categorized as a pesticide by the EPA. PZP “works” by tricking the immune system into attacking and destroying the ovaries. PZP has many adverse effects as well as unintended consequences. PZP presents safety-hazards to humans who handle it. PZP is a dangerous pesticide whose use is antithetical to the spirit and intent of the Wild and Free-Roaming Horses and Burros Act. BLM’s continuing to use PZP while ignoring and suppressing the evidence of its harmful effects constitutes malfeasance.

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This report was completed by Marybeth Devlin on December 24, 2015. Copyright Marybeth Devlin and Protect Mustangs 2015.

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References:
1. EquiMed staff. (2010, March 13) Equine Reproductive Maturity in Mares and Stallions. Puberty in Equines. Retrieved from http://equimed.com/health-centers/reproductive-care/articles/equine-reproductive-maturity-in-mares-and-stallions

2. EWCS. (2015, November 10). Re: “Contraceptive could reduce taxpayer costs for wild horses.” Retrieved from http://wyomingpublicmedia.org/post/contraceptive-could-reduce-taxpayer-costs-wild-horses#comment-2352628323

3. Ferguson, Mike. (2015, June 4) “Police called as group protests wild horse contraceptives.” The Billings Gazette. Retrieved from http://billingsgazette.com/news/state-and-regional/montana/police-called-as-group-protests-wild-horse-contraceptives/article_81462303-e128-5ee8-a7ef-2c8b098450f6.html

4. Gray, M.E. and Cameron, E.Z. (2010) Does contraceptive treatment in wildlife result in side effects? A review of quantitative and anecdotal evidence. Reproduction 139, 45-55. Online publication date: 1-Jan-2010. Retrieved at http://www.reproduction-online.org/content/139/1/45.full

5. Kaur, Kiranjeet and Prabha, Vijay. (2014) “Immunocontraceptives: New Approaches to Fertility Control,” BioMed Research International, vol. 2014, Article ID 868196, 15 pages, 2014. doi:10.1155/2014/868196. Retrieved from http://downloads.hindawi.com/journals/bmri/2014/868196.pdf

6. Kirkpatrick, J. F., I. K. M. Liu, J. W. Turner, Jr., R. Naugle, and R. Keiper. 1992a. Long-term effects of porcine zonae pellucidae immunocontraception on ovarian function of feral horses (Equus caballus). J. Reprod. Fert. 94:437-444. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1317449

7. Kirkpatrick, Jay. 2007. “Response to PA [Pennsylvania] Game Commission.” Posted on PNC’s Wildlife Forever Home Page. Retrieved from http://www.pzpinfo.org/home.html

8. Kirkpatrick, Jay F. (2015, May 16). Op-ed: Wild-horse contraceptives are based on sound science. The Salt Lake Tribune. Retrieved from http://www.sltrib.com/opinion/2517266-155/op-ed-wild-horse-contraceptives-are-based-on?fullpage=1

9. Knight, Colleen M., Rubenstein, Daniel I. 2014. The Effects of Porcine Zona Pellucida Immunocontraception on Health and Behavior of Feral Horses (Equus caballus). Princeton University Thesis, Ecology and Evolutionary Biology. Retrieved from http://dataspace.princeton.edu/jspui/handle/88435/dsp01vt150j42p

10. Nettles, Victor F. (1997) Potential consequences and problems with wildlife contraceptives. Reproduction, Fertility and Development 9(1) 137 – 144. Retrieved from http://www.publish.csiro.au/paper/R96054.htm Accessed full pdf text via purchase of a copy from Csiro Publishing.

11. Nuñez, Cassandra, Jim Adelman and Dan Rubenstein. (2015, July 3). Op-ed: Wild horse contraception not without unintended consequences. The Salt Lake Tribune. Retrieved from http://www.sltrib.com/opinion/2653298-155/op-ed-wild-horse-contraception-not-without

12. PCOS Foundation. (2015) What Causes PCOS? Retrieved from http://www.pcosfoundation.org/what-is-pcos

13. PNC, Inc. (Pity Not Cruelty). PZP FAQs. (2006) “Frequently Asked Questions on Immunocontraception.” (Special thanks to Jay Kirkpatrick and Rick Naugle for additions and corrections). Retrieved from http://www.pzpinfo.org/pzp_faqs.html

14. Raab. Lauren. (2015, May 31) “120,000 endangered saiga antelopes die mysteriously in Kazakhstan.” Los Angeles Times. Retrieved from http://www.latimes.com/science/sciencenow/la-sci-sn-saiga-antelope-die-off-20150531-story.html

15. Ransom JI, Hobbs NT, Bruemmer J (2013) Contraception Can Lead to Trophic Asynchrony between Birth Pulse and Resources. PLoS ONE 8(1): e54972. doi:10.1371/journal.pone.0054972. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/23383018

16. Rose-Cihakova-Caturegli Laboratory. (n.d.) Autoimmune Oophoritis. Johns Hopkins Pathology. Retrieved from http://pathology.jhu.edu/department/RCCLab/Oophoritis.cfm

17. Sacco AG, Subramanian MG, Yurewicz EC. (1981) Passage of zona antibodies via placenta and milk following active immunization of female mice with porcine zonae pellucidae. J Reprod Immunol. 1981 Dec;3(6):313-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7328557

18. Sussman, Karen. (2015 June 6) “Suspicious Deaths with Use of Anti-Fertility Drugs.” International Society for the Protection of Mustangs and Burros. Retrieved from http://www.ispmb.org/BirthControlDeaths.html

19. United States Department of the Interior. Integrity of Scientific and Scholarly Activities Policy. Code of Conduct. Retrieved from https://www.doi.gov/scientificintegrity

20. United States Environmental Protection Agency. Office of Chemical Safety and Pollution Prevention. Pesticide Fact Sheet. Porcine Zona Pellucida (PZP). New Chemical. Nonfood Use. January 2012. Retrieved from
http://www3.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf

21. U.S. Department of Health and Human Services. National Institutes of Health. Endocrine Disruptors. Retrieved from http://www.niehs.nih.gov/health/topics/agents/endocrine/

22. U.S. National Library of Medicine. National Institutes of Health. Ovarian overproduction of androgens. Retrieved from https://www.nlm.nih.gov/medlineplus/ency/article/001165.htm

23. Varras M, Anastasiadis A, Panelos J, Balassi E, Demou A, Akrivis CH. (2013) Autoimmune oophoritis: Clinical presentation of an unusual clinical entity. OA Case Reports 2013 Jan 31;2(1):7. Retrieved from http://www.oapublishinglondon.com/article/369#

Pm PZP Darts

 

www.ProtectMustangs.org
Protect Mustangs is a nonprofit organization who protects and preserves native and wild horses.




Despite protests, BLM returns wild horses to range in Nevada

Fish Creek Mares Indian Lakes aka Broken Arrow 2015

April 12, 2015

RENO, Nev. (AP) — Despite the protests of a rural county and rancher, the U.S. Bureau of Land Management has returned some 160 wild horses to the range in central Nevada.

The agency returned the horses to the Fish Creek Herd Management Area near Eureka on Tuesday after being cleared to do so by the Interior Board of Land Appeals.

The BLM originally had planned to return some 100 mares treated with a fertility control vaccine and 80 studs to the HMA on Feb. 20. They were among 424 horses removed from the HMA during a roundup that ended earlier in February.

The bureau routinely thins what it calls overpopulated herds on public land across the West, sending horses that aren’t adopted by the public to pastures in the Midwest for the rest of their lives.

The agency also routinely releases mares treated with fertility control drugs back to the range after being rounded up. Varying numbers of studs also are released back to the range to help maintain the genetic viability of herds.

Eureka County commissioners and rancher Kevin Borba filed an appeal with the Interior Board of Land Appeals to block the return of any of the 424 horses to the range and to challenge the BLM’s assessment of how many horses the HMA can support.

But the board affirmed the BLM’s authority to return 162 of the horses to the range. Arguments in the case continue on the underlying claims.

Borba has said the BLM has drastically reduced his livestock allotments in the HMA while allowing well over twice as many horses in it as it can support. He and Eureka commissioners seek the removal of more horses.

Horse advocates praised the BLM’s return of the horses to the range, saying it’s in line with recommendations released in 2013 by a National Academy of Sciences panel calling for increased emphasis on fertility control to keep horse numbers down.

“Now is the time to move forward with innovative management that makes sense, keeping wild horses on their range and saving millions of tax dollars in the long term,” Neda DeMayo, president of Return to Freedom, said in a statement. “It is time for a new direction instead of wasting time and money obstructing positive solutions that will benefit the horses, wildlife, ranchers and the range.”

But not all horse advocacy groups support the use of fertility control drugs on mares.

“We want to see drug-free holistic management used for native wild horses,” said Anne Novak, executive director of Protect Mustangs. “(The fertility control vaccine) PZP sterilizes after multiple use and we’re concerned that will ruin survival of the fittest.”

Borba has said he thinks the fertility control vaccine is far less effective than the BLM and horse advocates claim, and horse numbers will further explode as a result. Ranchers view wild horses as competition for scare forage in the arid West.

Cross-posted for educational purposes only from the San Francisco Chronicle.

WARNING: Slaughter Fear-mongering pushes forced drugging with PZP on wild horses!

PM HSUS Roundup

Helicopter roundups will increase to administer PZP

95% of the herds can’t be shot with darts in the field

Does the Humane Society of the United States (HSUS) want to take over the Wild Horse and Burro Program, pushing forced drugging with PZP (pesticide made from slaughterhouse pig ovaries http://www.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf) for management on underpopulated herds? PZP is a “tool” to manage wild horses to extinction. Science proves PZP (native, 22, etc.) sterilizes after multiple use.

Isn’t the American Wild Horse Preservation Campaign (AWHPC) the wild horse mouthpiece for HSUS? Doesn’t their pledge to “keep them in the wild” written up in the Huffington Post include forced drugging with PZP as their cornerstone management tool? Does HSUS want to create zoo-like settings to “keep them in the wild”? HSUS endorsed the former Secretary of Interior’s Plan, aka the Salazar Plan, in 2009. Now the plan is entering the final wipe-out phase with the help of PZP pushers.

For decades native wild horses have been used by the pharmaceutical industry as lab rats for birth control “research” at huge taxpayer expense. This involves killing wild horses to research drug damage on ovaries and other organs. PZP = Roundups = Cruel Animal Experiments

 

Links of interest™:

Salazar presents ambits plan to manage West’s wild horses (Washington Post, October 2009) http://www.washingtonpost.com/wp-dyn/content/article/2009/10/07/AR2009100703237.html  “Some animal advocates, including Wayne Pacelle of the Humane Society, praised the plan, but others decried it.”

Experimenting on wild horses creates cheap R & D for drug makers http://protectmustangs.org/?p=7993

Facebook Forum on PZP: https://www.facebook.com/groups/ForumPZPWildHorsesBurros/

 

BREAKING NEWS: Sudden protest against BLM censorship, wild horse roundups and using PZP (pesticide) to manage wild horses to extinction

PM Edita Cat

 

BLM refused to hear public comments at “public” meeting

MINDEN, NV (January 22, 2015)—Edita Birnkrant, Campaigns Director for Friends of Animals (FoA) flew out from New York City with FoA correspondent Nicole Rivard to give public comments at the Bureau of Land Management (BLM) public meeting about the Carson City District Draft Resource Management Plan which calls to zero out 6 treasured herds of wild horses. After being denied her rights at the public meeting, held at the Carson Valley Inn in Minden, Nevada this afternoon, Birnkrant took over the microphone at the BLM meeting and held up yellow crime scene tape while Rivard filmed the protest against censorship and managing wild horses to extinction. Birnkrant was threatened with arrest by Nevada Sheriffs while holding up her banner. The hotel manager made Rivard stop filming and told the advocates they were being thrown out of the hotel, even though they had booked rooms there that night.

 

Statement from Edita Birnkrant:

“While we were waiting to go into the meeting a man told a BLM staffer “I wanna open up a horse butcher shop”. Then a few other guys started making jokes about how tender horse meat is. The BLM guy just chuckled but didn’t tell them it was inappropriate.

I was outraged that the BLM dared to hold a “public ” meeting and forbid the public from speaking. I took over the microphone to call out the sham of a BLM meeting, that shut out the public, and I said that Friends of Animals was there tonight to oppose the BLM’s extinction plan for wild horses in Nevada. I said the BLM is managing wild horses to extinction through roundups and PZP and we are outraged and demand it stop. I held our banner that said “Stop the BLM’s Criminal Reign of Terror. Protect Wild Horses Under the Endangered Species Act” The sheriffs were surrounding me at that point threatening to arrest me unless I left. I still had the banner and was shouting “the BLM is charged with crimes against wild horses”.

Then the hotel manager at the Carson Valley Inn in Minden, Nevada—Phil Dohrn–started bullying us and got in Nicole’s face. He pushed against her—blocking the camera and told her she had to shut her video off and we were getting thrown out.

Three extremely hostile sheriffs and the Carson Valley Inn manager escorted us to our rooms and waited outside while we packed our bags. They pounded on the door to hurry us or they’d arrest us. They called additional sheriffs to the hotel during all this. We left the hotel shocked that the Carson Valley Inn treats paying guests who exercise their First Amendment rights in their meeting room like this.”

The federal plan for public land in the Reno/Carson area is of interest to all Americans from coast to coast. Citizens care about public land and want federally protected wild horses protected by the law that allows them to roam freely without harassment.

PZP is an EPA approved restricted-use pesticide (http://www.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf) that sterilizes wild mares after multiple use. Americans are learning about the dangers of PZP and are outraged the BLM would allow this to be used on wild horses.

Friends of Animals, an international animal protection organization founded in 1957, advocates for the rights of animals, free-living and domestic around the world. www.friendsofanimals.org

# # #

Friends of Animals’ public comments that advocates were not allowed to read and were given to Collen Sievers the BLM BLM Project Manager for Carson City District at the public hearing on the draft resource management plan for Carson City District

Edita Birnkrant, FoA’s campaigns director 917-940-2725

The opinion of the American public, as declared through Congress is clear: “wild free-roaming horses and burros are living symbols of the historic and pioneer spirit of the West; they contribute to the diversity of life forms within the Nation and enrich the lives of the American people.” BLM has an obligation to consider wild horses as an integral part of the natural system of public lands.

It appears from the Carson City’s Draft Resource Management Plan (RMP) and Environmental Impact Statement that the BLM failed to take into consideration critical information about wild horses and failed to consider any alternatives that promote a free and viable wild horse population.

Friends of Animal is here to urge BLM to reevaluate its Resource Management Plan.

We ask that BLM consider an alternative that: (1) maintains all wild horse herd management areas; (2) prohibits conflicting uses on herd management areas; and (3) prohibits efforts to eradicate wild horses, such as round-ups, fertility control and sterilization. BLM must take into consideration the small population of wild horses and the potential that they will be listed as a threatened or endangered species under the Federal Endangered Species Act. From a scientific perspective, wild horses on our public lands are at risk of extinction if BLM does not change its management plans.

BLM does not provide adequate area for wild horses. Under the current RMP, approximately 4.8 million acres of public lands covered by the plan are open for private ranchers to graze cattle and sheep while only 1.2 million acres are reserved for wild horses. In the preferred alternative the ratio or area available for cattle and sheep grazing is also more than 4 times that available for wild horses.

Moreover, under no alternative, are cattle and sheep prohibited from grazing on wild horse herd management areas. BLM must consider an alternative that provides contiguous habitat for wild horses to roam freely.

Second, all alternatives for the proposed Resource Management Plan allows BLM to continue managing horses at artificially low populations, or appropriate management levels. This results in expensive, and cruel round-ups that tear the wild horses from their homes and families and place them in tax funded holding facilities. This is one of the largest threat to wild horses on U.S. lands. Experts have warned that the “majority of wild equid populations managed by the BLM are kept at population sizes that are small enough for the loss of genetic variation to be a real concern.”

The Equid Specialist Group of IUCN Species Survival Commission recommends minimum populations of 2,500 individuals for the conservation of genetic diversity. Others have warned that populations managed with a target size of fewer than 500 horses are at some risk of losing more than 90 percent of selective neutral genetic variation over a period of 200 years.

There are no herds that have a large enough population to meet the recommendation of the IUCN Species Survival Commission – 2,500 animals—and only 1 out of 17 of the herd management areas in this planning area has an appropriate management level set to 500 or more. Limiting horses to an artificially low number is short-sighted and ineffective because it could prompt short-term population growth.

Finally, Friends of Animals submitted a petition to the US Fish and Wildlife Service asking it to recognize wild horses as threatened or endangered. The Endangered Species Act requires the government to make final determination on the petition within 12 months – which would be this June. The BLM should not undermine this legal process by allowing BLM to round-up and remove wild horses from Carson City herd management areas. Not only would such actions undermine the Endangered Species Act, but they would also put the viability of the horses here at risk. Instead the plan should recommend BLM halt all efforts to remove wild horses, and allow Fish and Wildlife Service to review the law and facts in regards to wild horses.
Nicole Rivard, correspondent, FoA 203-910-1217

As my colleague just pointed out, all but one of the 17 herd management areas in the Carson City District has an appropriate management level set to 500 or more. Everywhere else the loss of genetic viability is a real concern. So additional roundups, which destroy social structure that can lead to population spikes, as well as consideration of administering fertility control, should be removed from this Carson City District Plan immediately if not sooner.
While some wild horse advocates may claim fertility control drugs, such as PZP, is the lesser of two evils, we at FoA believe birth control is equally harmful and inhumane as roundups. In most cases—even the BLM admits this—wild horses would still have to be captured to be treated with the pesticide before being released.

The widespread use of PZP is really very contrary to the true core intent of the Wild Horse and Burro Act of 1971, which was to restore wild horses as naturally, integrated, harmonious components of the public land ecosystem who are not overly tampered with. Deciding which animal should give birth or not is a very invasive, unacceptable thing to do to these wild animals.

Studies have revealed adverse effects of PZP— that it sterilizes wild horses after multiple uses and results in risky foal birth out of season and significant behavioral changes that can affect the health of the herd.

BLM’s discussion regarding a population control program in the EIS is inaccurate and unsupported. They claim fertility control limits the stress of pregnancy on mares, and helps stallions as they will not be exerting extra energy fighting to control mares or raising foals.

What about the stress on mares of not being able to get pregnant as nature intended!

We urge the BLM to look beyond data provided by the Humane Society of the United States, which has a vested interest in PZP as it is the registrant of the pesticide, and Jay Kirkpatrick, the director of the Science and Conservation Center, which produces the active ingredient in PZP. For instance a 2009 Princeton University study of the horses on Shackleford Banks in North Carolina, who began getting PZP in 2000, showed that prolonged infertility has significant consequences on social behavior.

Researchers found that females who were receiving contraception were much more likely to change groups. Normally bands are really very stable, said researcher Cassandra Nunez, and mares will stay with males much if not all of their lives. That stability is really important for the health of the group members. Foal mortality increases when there are a bunch of different changes, and parasite load of animals in the group can go up because they are getting more stressed.

In a later study in 2010, Nunez found that recipients of PZP also extend the receptive breeding period into what is normally the non-breeding season, resulting in foal birth out of season.

Normally the winter is spent eating as much as they can, and everyone is more relaxed. Males tend to let females roam farther, which is good because food is patchier. So all of this is changing because of extended cycling.
Nunez also noted it’s taking a while for the contracepted mares, who were taken off PZP in 2009, to respond physiologically. So that flexibility that you think you have with PZP…it’s not really that flexible.”

It is imperative that BLM reduce the number of cattle and sheep allowed to graze on public lands, as well as consider holistic resource management plan, such as reserve design, which is described in detail in Craig Downer’s Book the Wild Horse Conspiracy. Both options would adequately protect these majestic animals so that they can persist for future generations.

Friends of Animals, an international animal protection organization founded in 1957, advocates for the rights of animals, free-living and domestic around the world. www.friendsofanimals.org

# # #

BLM Nevada News
CARSON CITY DISTRICT OFFICE NO. CCDO 2015-11
FOR RELEASE: November 28, 2014
CONTACT: Lisa Ross, 775-885-6107, lross@blm.gov

Draft Resource Management Plan Environmental Impact Statement Available for
BLM Carson City District

Carson City, Nev. – The Bureau of Land Management (BLM) is asking the public to review and comment on a Draft Resource Management Plan (RMP) and Environmental Impact Statement (EIS) for the Carson City District. The draft plan will affect approximately 4.8 million acres of public land. The comment period opened with the publication of a notice of availability in the Federal Register on November 28, 2014. Comments will be accepted during a 120-day period which closes March 27, 2015.

Public meetings to review and comment on the draft EIS will be announced at least 15 days in advance in local newspapers and on the BLM website.

The plan will address: Areas of Critical Environmental Concern, lands and realty, utility corridors, wind energy, travel management, recreation, fish and wildlife, minerals, wild and scenic rivers, public health and safety, and visual resource management.

Public meetings on the Draft RMP/Draft EIS are currently scheduled for 5:00 to 7:00 p.m.; on January 13, at the John Ascuaga’s Nugget (1100 Nugget Ave.) in Sparks, Nev.; on January 15, at the Fallon Convention Center (100 Campus Way) in Fallon, Nev.; on January 20, at the Mineral County Library (First & A Street) in Hawthorne, Nev.; on January 22, at the Carson Valley Inn (1627 US Hwy 395 N) in Minden, Nev.; and on January 29, at the Yerington Elementary School (112 N. California St.) in Yerington, Nev. An additional public meeting will be held from 2:00 to 4:00 p.m., on January 24, at the Carson City Plaza Hotel and Event Center (801 South Carson Street) in Carson City, Nev. Additional public meetings are anticipated in coordination with local County Commissions and Boards of Supervisors.

Written comments related to the Carson City District Draft RMP/Draft EIS may be submitted by any of the following methods:
• Website: http://on.doi.gov/1uYBNGT• E-mail: BLM_NV_CCDO_RMP@blm.gov
• Fax: 775-885-6147
• Mail: BLM Carson City District, Attn: CCD RMP, 5665 Morgan Mill Rd., Carson City, NV 89701.

Copies of the Carson City District Draft RMP/Draft EIS are available in the Carson City District Office at the above address or on the following website: http://on.doi.gov/1uYBNGT

Visit The Facebook Forum on PZP for more https://www.facebook.com/groups/ForumPZPWildHorsesBurros

#horses #animals #AnimalCruelty #FreeSpeech #Nevada #Tourism #WildHorses #mustangs #Tesla #money #energy #mining #water #yelp #hotel #travel #Reno #Tahoe #Sheriff #Carson #PZP #EPA #roundup #America

Equine reproductive immunology Ph.D speaks out in 2010 against using PZP on wild horses

PM President Obama Listen to the Science

November 27, 2010

Jared Bybee, Wild Horse and Burro Specialist

Department of the Interior

Bureau of Land Management

Billings Field Office

5001 Southgate Drive

Billings, Montana 59101-4669

VIA FAX: 406-896-5281

RE: Pryor Mountain Wild Horse Range Fertility Control Preliminary Environmental
Assessment Tiered to the Pryor Mountain Wild Horse Range Environmental Assessment and Herd Management Area Plan May 2009 EA DOI-BLM-MT-0010-2011-0004-EA

Dear Jared Bybee:

Background

I appreciate the opportunity to submit comments on the Pryor Mountain Wild Horse Range
Fertility Control Preliminary Environmental Assessment Tiered to the Pryor Mountain Wild
Horse Range Environmental Assessment and Herd Management Area Plan May 2009 EA DOI-BLM-MT-0010-2011-0004-EA. My background is in equine reproductive immunology and wildlife conservation. I applaud the Billings Field Office of the Bureau of Land Management (BLM) for a thoughtful approach to this issue. Cover letter 4700 (010.JB) dated November 2010 and signed by James M. Sparks, Field Manager states that the BLM would consider comments and revision to the EA or unsigned FONSI as appropriate. I urge a “no action alternative” as outlined on page 7 and 8 of the EA. This request is based on two pieces of new scientific evidence about effects of current immuno-contraception use.

Porcine Zona Pellucida (PZP) Contraception

The proposed action as stated on page 7 of this EA would exempt “mares ages 5-10 unless they have produced foals, or are part of a large bloodline.” This is reminiscent of the approach taken with the Assateague Island wild horse population. It is a compromise approach to this issue, in comparison to placing all mares on PZP. However a recent study shows that mitochondrial DNA diversity is low in the Assateague Island horse herd (Eggert et al. 2010). Since mitochondrial DNA is inherited from the mother (mare), this is evidence that female inherited genetics on Assateague Island wild horses is under represented. It is imperative that this be assessed before rolling out a similar management plan for the Pryor Mountain wild horses.

There is a recent Princeton University study on PZP effects. Consecutive PZP applications, analogous to the proposed action plan in this EA, showed that mares gave birth later in the season, and were cycling into the fall months (Nunez et al. 2010). In a state like Montana where freezing temperatures are found in the fall, this can have serious and long term effects on foal survivorship.

I must include a statement on long term consecutive use of PZP. Any form of PZP contraception is not completely reversible in mares depending on the length of use of PZP. Contraception can only be reversed when the antibody titer decreases to 50-60% of the positive reference sera (Liu et al. 2005). Mares treated for 7 consecutive years do not return to viable fertility (Kirkpatrick and Turner 2002; Kirkpatrick et al. 2009). The issue of reversible contraception is very important to be able to maintain wild equines in the United States. Long term treatment with PZP has inherent negative potential for this herd.

I am requesting a new look at the proposed fertility control action for the Pryor Mountain wild horses.

Sincerely,

Christine DeCarlo, Ph.D.

Lori S. Eggert, David M. Powell, et al. (2010). “Pedigrees and the Study of the Wild Horse
Population of Assateague Island National Seashore.” Journal of Wildlife Management
74(5): 963-973.

J. F. Kirkpatrick, A. Rowan, et al. (2009). “The practical side of immunocontraception: zona
proteins and wildlife.” J Reprod Immunol 83(1-2): 151-7.

J. F. Kirkpatrick and A. Turner (2002). “Reversibility of action and safety during pregnancy of immunization against porcine zona pellucida in wild mares (Equus caballus).” Reprod
Suppl 60: 197-202.

I. K. Liu, J. W. Turner, Jr., et al. (2005). “Persistence of anti-zonae pellucidae antibodies
following a single inoculation of porcine zonae pellucidae in the domestic equine.”
Reproduction 129(2): 181-90.

Cassandra M. V. Nunez, James S. Adelman, et al. (2010). “Immunoctraception in Wild Horses (Equus caballus) Extends Reproductive Cycling Beyond the Normal Breeding Season.” PLos ONE 5(10): 1-10.

(Posted for educational purposes)

 

Equine reproductive immunology Ph.D speaks out in 2010 against using PZP on wild horses

Native Wild Horses (Photo © Cynthia Smalley, all rights reserved)

Native Wild Horses (Photo © Cynthia Smalley, all rights reserved)

November 27, 2010 

Jared Bybee, Wild Horse and Burro Specialist 

Department of the Interior 

Bureau of Land Management 

Billings Field Office 

5001 Southgate Drive 

Billings, Montana 59101-4669 

VIA FAX: 406-896-5281 

RE: Pryor Mountain Wild Horse Range Fertility Control Preliminary Environmental 
Assessment Tiered to the Pryor Mountain Wild Horse Range Environmental Assessment 
and Herd Management Area Plan May 2009 EA DOI-BLM-MT-0010-2011-0004-EA 

Dear Jared Bybee: 

Background 

I appreciate the opportunity to submit comments on the Pryor Mountain Wild Horse Range 
Fertility Control Preliminary Environmental Assessment Tiered to the Pryor Mountain Wild 
Horse Range Environmental Assessment and Herd Management Area Plan May 2009 EA DOI-
BLM-MT-0010-2011-0004-EA. My background is in equine reproductive immunology and 
wildlife conservation. I applaud the Billings Field Office of the Bureau of Land Management 
(BLM) for a thoughtful approach to this issue. Cover letter 4700 (010.JB) dated November 1. 
2010 and signed by James M. Sparks, Field Manager states that the BLM would consider 
comments and revision to the EA or unsigned FONSI as appropriate. I urge a “no action 
alternative” as outlined on page 7 and 8 of the EA. This request is based on two pieces of new 
scientific evidence about effects of current immuno-contraception use. 

Porcine Zona Pellucida (PZP) Contraception 

The proposed action as stated on page 7 of this EA would exempt “mares ages 5-10 unless they 
have produced foals, or are part of a large bloodline.” This is reminiscent of the approach taken 
with the Assateague Island wild horse population. It is a compromise approach to this issue, in 
comparison to placing all mares on PZP. However a recent study shows that mitochondrial DNA 
diversity is low in the Assateague Island horse herd (Eggert et al. 2010). Since mitochondrial 
DNA is inherited from the mother (mare), this is evidence that female inherited genetics on 
Assateague Island wild horses is under represented. It is imperative that this be assessed before 
rolling out a similar management plan for the Pryor Mountain wild horses. 

There is a recent Princeton University study on PZP effects. Consecutive PZP applications, 
analogous to the proposed action plan in this EA, showed that mares gave birth later in the 
season, and were cycling into the fall months (Nunez et al. 2010). In a state like Montana where 

freezing temperatures are found in the fall, this can have serious and long term effects on foal 
survivorship. 

I must include a statement on long term consecutive use of PZP. Any form of PZP contraception 
is not completely reversible in mares depending on the length of use of PZP. Contraception can 
only be reversed when the antibody titer decreases to 50-60% of the positive reference sera (Liu 
et al. 2005). Mares treated for 7 consecutive years do not return to viable fertility (Kirkpatrick 
and Turner 2002; Kirkpatrick et al. 2009). The issue of reversible contraception is very important 
to be able to maintain wild equines in the United States. Long term treatment with PZP has 
inherent negative potential for this herd. 

I am requesting a new look at the proposed fertility control action for the Pryor Mountain wild 
horses. 

Sincerely, 

Christine DeCarlo, Ph.D. 

Lori S. Eggert, David M. Powell, et al. (2010). "Pedigrees and the Study of the Wild Horse 
Population of Assateague Island National Seashore." Journal of Wildlife Management 
74(5): 963-973. 

J. F. Kirkpatrick, A. Rowan, et al. (2009). "The practical side of immunocontraception: zona 
proteins and wildlife." J Reprod Immunol 83(1-2): 151-7. 

J. F. Kirkpatrick and A. Turner (2002). "Reversibility of action and safety during pregnancy of 
immunization against porcine zona pellucida in wild mares (Equus caballus)." Reprod 
Suppl 60: 197-202. 

I. K. Liu, J. W. Turner, Jr., et al. (2005). "Persistence of anti-zonae pellucidae antibodies 
following a single inoculation of porcine zonae pellucidae in the domestic equine." 
Reproduction 129(2): 181-90. 

Cassandra M. V. Nunez, James S. Adelman, et al. (2010). "Immunoctraception in Wild Horses 
(Equus caballus) Extends Reproductive Cycling Beyond the Normal Breeding Season." 
PLos ONE 5(10): 1-10.

(Posted for educational purposes)