Poppy (#1196) deserves to live in safety

PM Poppy #1196 3-Strike PVC FB

Poppy (#1196) is from the Silver King Herd Management Area (HMA) in Nevada. She was 5-years-old when she was rounded up to never have any sense of freedom or family again.

This American mustang survived in the wild as a foal when so many perish due to natural causes. Life in the wild is harsh and only about 50% of the foals born will live to be 2-years-old. This is survival of the fittest and exactly what makes American mustangs such a strong and special breed.

Now Poppy is 7 and has been help captive for 2 years without shade or shelter . . .

Please Help Poppy! She has been passed over in 3 adoptions because her photos are bad. That shouldn’t ruin her life. It’s not her fault.

By sharing this post on Facebook, Twitter and by email you can help Poppy find her forever home. She is for sale for $25 and she is wild. That means she will need to go to a trainer for gentling or her new person would need to borrow a high fenced round pen and gentle her like so many people do–with LOVE and patience.

Together we can make this happen!

BoLM says:

Sex: Mare Age: 7 Years   Height (in hands): 14

Necktag #: 1196   Date Captured: 11/11/14

Freezemark: 09621196   Signalment Key: HF1CAAEAD

Color: Brown   Captured: Silver King (NV)

Notes:

Tag-#1196. 7 year old brown mare gathered from the Silver King Herd Management Area in Nevada in November of 2014.

Sale information is here: http://www.blm.gov/wo/st/en/prog/whbprogram/adoption_program/sales.html

Update August 10: BLM said, “If no bids were placed on an animal in the last internet and a bidder that didn’t get the horse they choose as first pick didn’t decide to take a horse with no bid then those horses with no bids are available for pickup at PVC till August 22. After that date any remaining horses will be put on the next internet adoption. . . horses are available for pick up FROM PVC ONLY we will not ship as the truck is full at this point.”

This horse is currently located in Palomino Valley, NV.  For more information, please contact Jeb Beck at (775) 475-2222 or e-mail: j1beck@blm.gov

Stop the Roundups for Experiments, Pesticide PZP and removals!

Protect Mustangs is an organization who protects and preserves native and wild horses.




Groups who want Pesticide PZP used on America’s last wild horses

 

According to a press release by Return to Freedom Sanctuary, who seems to have received money from the BLM in the past, the following groups want to use PZP on wild horses:

Alliance of Wild Horse Advocates

American Society for the Prevention of Cruelty to Animals

American Wild Horse Preservation Campaign

Animal Legal Defense Fund

Animals Voice

Animal Welfare Institute

Black Hills Wild Horse Sanctuary

Center for Animal Protection and Education

Citizens Against Equine Slaughter

The Cloud Foundation

Corolla Wild Horse Fund

Friends of a Legacy

Front Range Equine Rescue

Habitat for Horses

Hidden Valley Wild Horse Protection Fund

Horses for Life Foundation

Humane Society of the United States

Jicarilla Mustang Heritage Alliance

Least Resistance Training Concepts

Lifesavers Wild Horse Rescue

Montgomery Creek Ranch

National Mustang Association, Colorado Chapter

Oregon Wild Horse & Burro Association

Photographers for the Preservation of Wild Horses and Burros

Pine Nut Wild Horse Advocates

Respect 4 Horses

Return to Freedom Wild Horse Sanctuary and Preservation

Salt River Wild Horse Management Group

Serengeti Foundation

Southern Sun Farm Sanctuary

Steadfast Steeds

Virginia Range Wildlife Protection Association

Wild Equid League (Colorado)

Wild Horses of America Foundation

Wild Horse Connection

Wild Horse Education

Wild Horse Observers Association

Wild Horse Preservation League

Pm PZP Darts

 

PZP = Slow Extinction

 

Don’t let yourself be fear mongered. Read the Science Against PZP:

The Fact Sheet on PZP: http://protectmustangs.org/?p=8749

PZP is Dangerous: http://protectmustangs.org/?page_id=6922

 

PM No Evidence Overpopulation

Protect Mustangs is an organization who protects and preserves native and wild horses.




Did you join the group requesting Nazi-like sterilization experiments on pregnant wild mares?

© EquineClinic.comn shared for educational purposes

© EquineClinic.comn shared for educational purposes

See the list of sterilization activists who are asking for sterilization experiments on wild mares below

A group of Pro-Experiment activists on a Bureau of Land Management (BoLM) support Facebook page called Wild Horses, BLM and Logical Solutions (https://www.facebook.com/groups/1446611602254365/) , have asked the BoLM to experiment on wild horses. They wrote a letter calling for the Oregon sterilization experiments. They also asked for PZP to be used on the more tame herds. Pro-Experiment activists in their group signed it. Pro-Slaughter activists signed it too.

If you have “joined” their group–just to watch what this treacherous group of Pro-Slaughter, Pro-Experiment, Pro-Livestock, Pro-Pesticide PZP activists, BoLM employees and supporters, etc. are up to–know that they count all their group members as people supporting their agenda for sterilization experiments on wild pregnant mares in Oregon and elsewhere.

Recently one of their admins boasted, “We have 2,000 members . . . “.

We were very shocked to see The Cloud Foundation board member, Linda Gresham Hanick, vocal in the Wild Horses, BLM and Logical Solutions group but since this group not only pushes for sterilization experiments on pregnant mares but also pushes for Pesticide PZP–like the Cloud Foundation who calls and partners with BLM for Pesticide PZP–we understand why their board member might be there. (http://www.blm.gov/style/medialib/blm/mt/main_story.Par.31432.File.dat/TopStoryHorse.pdf)

Hanick seems to have been also a vocal member of a group Facebook shut down for Harassment and Hate Speech targeting our volunteer executive director, Anne Novak who created the Forum on PZP for Wild Horses & Burros on Federal Land (https://www.facebook.com/groups/ForumPZPWildHorsesBurros) educating thousands of people on Pesticide PZP. Novak is against experimenting on wild horses, against horse slaughter and a strong advocate for wild horse freedom often quoted in the news.

I Hate Group Reviewed by FB and Closed screen Shot 2016-05-06 at 12.29.26 AM

Many of the signors of the Ovarian Ligation and PZP letter were active members of a public group Facebook shut down for Harassment and Hate Speech targeting Anne Novak. Keep in mind Novak and other members of the Alliance for Wild Horses and Burros have been speaking out against the experiments since they announced them.

We have evidence of members of the Hate Group engaging in stalking, harassment, hate speech, etc. plotting to interfere with Anne Novak’s work, Protect Mustangs‘ mission as we as the mission of The American Wild Horse Institute, care of the wild horses rescued back from the slaughterhouse known as the Wyoming 14™ (WY14™) plus others and evidence of their plot to smear Novak’s good reputation and more.

Below are the names of the members of Logical Solutions who signed the letter calling for sterilization experiments on pregnant mares:

Proposal of Ovarian Ligation
By Sandee Force on Monday, August 24, 2015

From: Members of Wild Horses, BLM and Logical Solutions

To: U.S. Wild Horse and Burro Advisory Council

Re: Population Management of wild herds on HMAs

The members of the Wild Horses, BLM and Logical Solutions have spent time considering potential solutions to the ever increasing number of horses and burros needing to be removed from the range held in both Long Term Holding and Short Term Holding. We feel that a two pronged program would both greatly decrease the number of animals needing to maintained in this manner and allow older mares to live out their lives on the range.

We would like to suggest that ovarian ligation be put into an immediate test program in at least 2 and preferably 3 herds using herds that are widely watched and recorded by regional photographers. Our suggestion would allow for mares that are old enough to have had at least 4-5 foals accessed at gathering and removed to the closest holding facility to do ovarian ligation by a veterinarian who has experience in this procedure. We would suggest that working with the state veterinary school located closest to each facility would be the optimal way to get young vets trained on this procedure and to potentially hire vets specifically for the program from this pool of trained professionals.We realize that standard policy would normally be to spend a number of years doing in pen trials with horses that would be scheduled to go to LTH. We feel that this can be bypassed by using herds that are currently being observed and by training the photographers to record information on these mares that would give accurate information about how they assimilate back into the herds. We would like to suggest that along with the ovarian ligation all fillies 3 and under be given PZP and allowed to be more mature at first foaling.

To summarize our proposal as accurately as possible, allowing for changes needed by region or herd.

1. Two to three test herds be chosen that mares will be brought in and those 3 and under be given the correct dosage of PZP for their age and mares that are of an age to have 4-5 foals on the ground have the ovarian ligation procedure done to at the holding
facility. Those mares that are operated on can be held for an appropriate period for recovery at the facility to document reaction and to ensure proper healing of all incisions before being returned to the area that they were captured. Any foals that are under weaning age should stay with the mare in the holding facility and be release with her. Use a
small hip brand to designate ovarian ligation for observation purposes.

2. Any mare that shows a major genetic defect or has thrown multiple foals with genetic disorders should automatically be put into the ovarian ligation program no matter what the age.

3. Train photographers and volunteers to work with the USGS and Universities to properly document range interaction of both the mares who have been given ovarian ligation and those fillies given PZP. Video and photographic documentation of herd/band interaction would be ideal. It is imperative to have USGS and at least one University involved in both
documentation and study of the effect of ovarian ligation on herd dynamics and the health and well being of both, mares and foals as well as the local bands that they belong to.

4. Document the short and long term consequences of ovarian ligation on the mares, i.e. heath, longevity, and acceptance/position within the band. Note if the mares are removed from the bands and act like bachelor stallion bands.

5. Within 3 years if the results of the test herds are good expand to other BLM managed herds with the goal to cut down on required gathers to once every 6-8 years.

6. Look into the possibility of darting with PZP every 2 years to expand the time young mares have a chance to mature before starting to foal.

The goal of this plan is to decrease the rate of population growth on the range.
In conjunction it would allow these older mares to stay on the range without adding to population growth until their deaths and not have to be gathered and shipped to Long Term Holding Facilities for their senior years. Between the ovarian ligation and using PZP on the fillies the herd’s rate of growth could be reduced by 50% per year. This would substantially help both the range and the cost of gathering and housing the horses and burros while keeping more horses on the range. By hip branding the mares that have had ovarian ligation you would be able to gate cut those mares back onto the range at any subsequent gather and not have to haul them off the range.

Some of the herds suggested for this procedure are South Steens, Oregon; Sand Wash Basin, Colorado; Twin Peaks, California; and/or BLM HMA around the Reno/Carson area of Nevada. These are herds that have been previously documented and in the case of both Sand Wash Basin and South Steens there is photographic documentation of the herds for 5-7 years that would be available to work within this project.

Respectfully
Submitted,

Sandra Force – Junction City, Oregon
AJ Sutton- Lawndale,Ca.
Kari Masoner – Tuson, Arizona
Ana Andrick – Wellington, Colorado
Nancy Warrick Kerson – Napa, California
Kathleen T. Granzow – Genoa City, Wisconsin
Thomas P. Brunshilde – Hammond, Wisconsin
Karen Goodroad – Pleasant Hill, Oregon
Lea Erwood – Rosedale, IN.
Kathryn Shirley – Holly Springs, North Carolina
Margaret Rothauge (Maggie) Creswell, Oregon
Angela Robey – Witch Well, AZ
Tom Hool – Casper, Wyoming
Debbra Dotson Christensen – Coquille, Oregon
Stephanie Jones – Eugene, Oregon
Jamie M. Adkins – Casper, Wyoming
Lisa Sink-Sheridan, Oregon
Beverly Shaffer – Burns, Oregon
Ramona Bishop – Burns, Oregon
Shyla Creasey – Oregon
Stacey Harnew –
Andi Harmon – Burns, Oregon
Keelyn Fawcett – Salem, OR
Kimberly Omnes
Mark Omnes
Angela “Angel” Rakestraw – Dinwiddie, Va
Jennifer Gregton – Midvale, Idaho
Iris Benson – Corvallis, Oregon
Karen Landis – Centralia, WA
Candy Nichols – Poolville, TX
Christina Picchi
Bree Alsman – Sandy, Oregon
JoAnna Lamb – Boardman, Oregon
Tracey Westbury – Bellingham, Washington
Cathy Smith – Pleasant Hill, Oregon
Rhonda Chayer – Barton, Vermont
Debbie Jackson – Ellensburg, Washington
Jes Sothern – Oregon
Rex Moore – Denton, Texas
Rose Howe – Monument, Oregon
Kerry O’Brien – Van Nuys, CA.
Susan Clogson – Woodinville, Washington
Nancy Willard – Eugene, Oregon
Loretta M. Jones – Redmond, Oregon
Jennie Kreutzer – Arlington, Washington
Monica Shifflet – New Haven, PA
Crystal Cooke – Clovis, New Mexico
Christie Brown – Daphne, Alabama
Pat Garcia – Burnet, Texas
Carrie Marie Fuesler – Brownsville, Oregon
Jackie Mousseau – Clinton Township MI
Betty Forman
Kathy Tellechea – Lexington, OR
Jim Bishop – Hines, Oregon
Angela Huston – St Louis, Missouri
Mike Huston – St Louis, Missouri
Kay Hamilton – Phoenix, OR
Richelle Wilson – Hillsboro, OR
Suzanne Ganazzi – Point Reyes Station, California
Tina Smith – Sommers, Conn
Andrea Walker – Fort Worth, Texas
Jeni Adler Snyder, Oklahoma
Ash Michael – Madison, South Dakota
Ashley Lawler
Brigid Piccaro – Acton, California
Kathryn Meyer – Orion, MI
Nancy Kohl – Surprise, Arizona
Jeni Adler – Snyder, Oklahoma
Kate Bogel – Howell, New Jersey
Lara Mogensen – Ellensburg, Washington
Carol Davis – Selma, Oregon
Susan Humphrey – Hot Springs, South Dakota
Gini Everts – Eugene, Oregon 

Protect Mustangs is an organization who protects and preserves native and wild horses.




#URGENT: File a complaint against Nazi-like population control experiments on America’s wild horses!

The clock is ticking. Oregon State University isn’t stopping. They are going ahead with their Nazi-like population control experiments on wild mares and a lot of them are pregnant! The experiments were encouraged by a bunch of sick pro-slaughter, pro-cattle activists that work in darkness to bring the “final solutions” to America’s underpopulated wild horses and burros. These people have no soul. They have no empathy for the suffering these horrible experiments will inflict on WILD horses . . . wild animals . . . wildlife . . . that the law was supposed to protect.

Take action right now and fill out this Animal Welfare Complaint: https://www.aphis.usda.gov/aphis/ourfocus/animalwelfare/complaint-form. Mention that the procedures used to sterilize wild horses in the experiments at Oregon State University and elsewhere are cruel. Let them know that wild horses are underpopulated and the basis for these heinous experiments is false. Underpopulated wild horses and burros in America don’t need population control or birth control.

America’s wild horses and burros need your help to live and survive on public land set aside for them in 1971–the public sanctuary that is open 24/7 at no charge. The wild ones need YOU to go to your elected officials’ home offices and push for their protection.

Please also send an email to your congressman/woman and your 2 senators. Their contact information is here: http://www.contactingthecongress.org/ Short handwritten letters have the most impact as elected officials see them representing the opinion of 1000 voters.

Ignore anyone who says birth control is a tool in the stupid toolbox. Who’s toolbox are they talking about?

Ignore the spin doctors claiming they are overpopulated. It’s a lie.

The Bureau of Land Management’s (BoLM) claim there are 67,000 wild horses and burros combined in all western states is based on no more than an inflated guess. The BoLM have no headcount and no evidence according to the National Academy of Sciences. Even if there were 67,000 wild horses and burros left in the wild that would be too few to survive serious changes in climate, disease and environmental disaster. Do you want to see our majestic symbols of freedom and the American spirit become extinct forever? No you don’t, so take action. Your voice counts.

Don’t get distracted from the facts:

America’s wild horses and burros are being wiped off public land because greedy people and corporations with no conscious want to exploit the wild ones’ territory for profit–big profit. These people who are out for big money need to find the win-win, respect the environment and learn to work with–not annihilate–the last free roaming wild horses and burros. After all, wild horses prevent wildfires that could hurt their money making projects (oil and gas wells, solar energy zones, mining, etc.) These people should realize cattle will never roam like wild horses do and therefore cannot replace the wild herds for fire prevention.

Stop BLM from EXPERIMENTING on wild mares!

Tell your friends, family, co-workers and neighbors what’s going on and tune into our website daily for updates on the fight to save America’s wild horses! www.ProtectMustangs.org There are a lot of ways you can help by using your voice right from your computer. If we all don’t do something now then hundreds of wild horses will be cruelly carved up in Nazi-like population control experiments to rid the land of wild horses. Our beloved wild ones have been wrongfully labelled “pests” in the Pesticide PZP EPA application. Also based on the overpopulation lie, thousands of wild horses could end up at slaughter soon if they are not all accounted for and placed in safe homes.

Your elected officials need to be contacted regularly by email, handwritten letters and in meetings to stop the abuse against wild horses and keep them living in real freedom. . . in the wild.

It’s time to send an email requesting an appointment to get your elected officials involved in protecting America’s iconic wild horses and burros. Yes really protect them–not forcibly drug them with Pesticide PZP or sterilize them.

Do you realize YOUR voices in government have been fed a bunch of lies based on a false premise from other elected officials, lobbyists and traitor “advocates” as well? Follow the money . . . Then move beyond that to real “solutions” to protect real freedom. Make your voice heard.

From the Team at Protect Mustangs
www.ProtectMustangs.org

a member for the Alliance for Wild Horses and Burros

Protect Mustangs is an organization who protects and preserves native and wild horses.




Lies, Spin and Subterfuge

The Cattlemen and livestock industry are making a huge push in Washington to give millions of tax-dollars to BLM for HUGE roundups to sterilize America’s last wild horses and burros. That means they will die out. Watch the Video testimonies here:


The House Committee on Natural Resources is stacked against wild horses and burros.

The vet called to testify seems to be from a wealthy Nevada ranching family. The Farm Bureau witness seems to be Pro-Slaughter for wild horses, etc.

The only representative for wild horses testifying is pushing for cooperative agreements with BLM as well as fertility control–Pesticide PZP–and that opens a pandora’s box for all forms of fertility control based on the BLM myth that wild horses are overpopulated : (

The Oil and Gas industry is about to launch a huge #Fracking boom in the areas where wild horses live so follow the money behind this rigged hearing. . .

PUBLIC comment allowed for 10 Business days after this meeting, which is July 7, 2016.
EMAIL: the Clerk: aniela.butler@mail.house.gov

This is YOUR call to action to JOIN the Alliance for Wild Horses and Burros to stop the slaughter, sterilizations, and stop the killing of America’s wild horses and burros. Go to https://www.facebook.com/Alliance-for-Wild-Horses-and-Burros-282933648725820/

Sign and share the petition for a 10 year Moratorium on Roundups: https://www.change.org/p/president-of-the-united-states-urgent-grant-a-10-year-moratorium-on-wild-horse-roundups-for-recovery-and-studies

Sign and share the petition to defund and stop the roundups as well as the slaughter: https://www.change.org/p/defund-and-stop-the-wild-horse-burro-roundups

Thank you!

From,

the Team at Protect Mustangs
(www.ProtectMustangs.org)
Facebook: https://www.facebook.com/ProtectMustangs

Member of the Alliance for Wild Horses and Burros (AWHB)
Facebook: https://www.facebook.com/Alliance-for-Wild-Horses-and-Burros-282933648725820/

 

Where are wild horses?™

 

Protect Mustangs is an organization who protects and preserves native and wild horses.




Is your representative in Congress pushing for America’s wild horses to lose their federal protections?

Stop elected officials who want to ruin the 1971 Free-Roaming Wild Horse and Burro Protection Act!
READ the shocking letter written last year by elected officials who seem to be supported by special interests groups: http://www.wylr.net/guest-opinions/347-letters-to-the-editor/5887-letter-to-the-editor-from-u-s-senators-and-representatives
 
Notice the following points in their letter:
 
These elected officials call the wild mustangs just “horses” most of the time not “wild horses”. Obviously, this is a constructed subliminal move to wipe out the WILD ones that are to be protected since 1971. [Note: Be sure to always refer to mustangs as wild horses]
 
Members of Congress, elected by the public but who seem to serve special interests, seem to make false claims of: “overstocking” in HMAs, failure to “dispose” of horses and burros, significant ecological “damage” to riparian areas, “overgrazing” and “compromised water” resources, etc.
 
We know the public has been telling these elected officials the truth for years so why aren’t they listening? Are they getting paid off?
 
They also claim “adoptions have “fallen almost 70 percent” in the last 10 years. Is this true?
Is this why the BLM makes it so hard to adopt wild horses due to the worst customer service in America? Do they want the adoption program to fail so they can kill them all?
 
The BLM always wanted to “dispose” of our cherished wild horses & burros. Sterilization is the next best thing in their eyes. They NEVER wanted to use PZP. They label return-native wild horses as “INVASIVE SPECIES” aka PESTS as you see in their letter. Just like the PZP Pesticide applicant classified them in their 2012 PZP application.
 
The signers of the letter seem to falsely claim: “Improper management compromises equine health, habitat conservation efforts and allows for resource degradation and encroachment by invasive species that will affect wildlife, livestock producers and recreationalists for decades to come.”
What about the cattle and sheep at more than 100 head of livestock to 1 wild horse that is grazing on public land? Do they think the American public is so stupid to buy into the myth that range degradation is the fault of wild horses?
Contact your elected officials across America to let them know you want your voice in Congress to stand up for what’s right, stand up for the 1971 law, keep America’s wild horses federally protected and never give them to the states!
 
The letter to Neil Kornze, the Director of BLM, was signed by the following elected officials from the Republican Party:
 
Sens. John Barrasso (R-Wyo.)
 
Mike Crapo (R-Idaho)
 
Steve Daines (R-Mont.)
 
Mike Enzi (R-Wyo.)
 
Jeff Flake (R-Ariz.)
 
Orrin Hatch (R-Utah)
 
Dean Heller (R-Nev.)
 
Mike Lee (R-Utah)
 
John McCain (R- Ariz.)
 
James Risch (R-Idaho)
 
Reps. Cynthia Lummis (R-Wyo.)
 
Mark Amodei (R-Nev.)
 
Jason Chaffetz (R-Utah)
 
Paul Gosar (R-Ariz.)
 
Raul Labrador (R-Idaho)
 
Steve Pearce (R-N.M.)
 
Adrian Smith (R-Neb.)
 
Chris Stewart (R-Utah)
 
Ryan Zinke (R-Mont.)
Now in April 2016 Rep Chris Stewart’s plan has gained momentum as you see in the video below
https://www.youtube.com/watch?v=-uPFMAE49NM
 
Links of interest:
Letter to BLM’s Neil Kornze asking for the states to grab control of America’s federally protected wild horses & burros: http://www.wylr.net/guest-opinions/347-letters-to-the-editor/5887-letter-to-the-editor-from-u-s-senators-and-representatives#sthash.allOgrdU.dpuf
PZP Application calls wild horses and burros “PESTS” to get the pesticide approved: https://www3.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf
READ the Associated Press article from 2014: Rep. Chris Stewart’s bill seeks to allow states to manage wild horses http://www.deseretnews.com/article/765656593/Bill-seeks-to-allow-states-to-manage-wild-horses.html

Protect Mustangs is an organization who protects and preserves native and wild horses.




FACT SHEET: The Truth about PZP

PM PZP Dr liu

 

EXPANDED SUMMARY OF PZP’S ADVERSE EFFECTS, INCLUDING REFERENCES

PZP — The Pesticide

Porcine zona pellucida (PZP aka ZonaStat-H or Native PZP) is an EPA-registered pesticide derived from the ovaries of slaughtered pigs. PZP is approved for use on wild horses “in areas where they have become a nuisance ….” [20]

Some persons argue that, because PZP does not kill the mare, it is not really a “pesticide.” Actually, PZP does kill. As will be documented in this report, its use is associated with stillborn foals. In the long term, PZP will weaken a herd immunologically, which could swiftly lead to its extinction. So, yes, PZP is a real pesticide.

PZP — an Anti-Vaccine

While touted as a “vaccine,” PZP is actually a perversion of what a true vaccine is supposed to be. Instead of preventing disease, PZP causes disease — auto-immune disease. Thus, PZP is an anti-vaccine.

PZP’s Mode of Action as Stated in the Pesticide Registration Is a Disproved Hypothesis

The registrant of PZP advised the Environmental Protection Agency (EPA) that, based on information from the pesticide’s researcher-manufacturer, PZP works by generating antibodies that “block sperm attachment.” This representation of PZP as a sort of chemical condom was not fact but merely an untested hypothesis, postulated three decades ago. The old hypothesis was disproved by subsequent research. PZP’s manufacturer knew, or should have known, this. The manufacturer should also have been informed and up-to-date regarding the side effects and unintended consequences of PZP. Yet, the manufacturer continued to cite the disproved hypothesis and to deny that PZP has any adverse effects. [7 and 13]

PZP’s True Mode-of-Action

So how does PZP really work? PZP tricks the immune system into waging war on the ovaries. In a meta-analysis of ZP-type contraceptives, Kaur & Prabha (2014) reported that the infertility brought on by such products is ” … a consequence of ovarian dystrophy rather than inhibition of sperm-oocyte interaction.” Thus, PZP’s antibodies “work” not by blocking sperm attachment but by destroying the ovaries. Kaur & Prabha further disclosed that ” … histological examination of ovaries of immunized animals revealed the presence of atretic follicles with degenerating oocytes.” [5] [Atretic follicles are ovarian follicles in an undeveloped state due to immaturity, poor nutrition or systemic disease; manifested by prolonged anestrus.]

Kaur & Prabha’s review concluded that PZP’s antibodies induce ovarian dystrophy, oophoritis (inflammation of the ovaries), destruction of oocytes in all growing follicles, and depletion of resting follicles. The manufacturer of PZP as well as Bureau of Land Management (BLM) should have been aware of these and other findings about the pesticide. Yet they ignored or disregarded any information that was contrary to their personally-preferred but obsolete and false description of PZP’s mode-of-action.

PZP Manufacturer’s Own Research Found Markedly Depressed Estrogen Secretion

In a telling study published back in 1992, the manufacturer of Native PZP, along with colleagues, reported that ” … three consecutive years of PZP treatment may interfere with normal ovarian function as shown by markedly depressed oestrogen secretion.” [6] Thus, despite all the hype about PZP being non-hormonal, the manufacturer knew that ZonaStat-H has an adverse hormonal effect, causing significantly-lowered estrogen. Thus, PZP is an endocrine disruptor. [21] The plummeting estrogen-levels may also reflect the ovarian dystrophy and oophoritis now known to be caused by PZP. Despite personally discovering negative hormonal impacts 23 years ago, PZP’s manufacturer continued to cite misinformation regarding the product’s mode-of-action and endocrine-disruptor side-effects.

PZP Causes Ovarian Cysts

In their 2010 meta-analysis, Gray & Cameron cited a number of studies that found ” … alterations to ovarian function, oophoritis, and cyst formation with PZP treatment (Mahi-Brown et al.1988, Sehgal et al. 1989, Rhim et al. 1992, Stoops et al. 2006, Curtis et al. 2007).” [4] These findings support those of Kaur & Prabha while introducing yet another adverse effect: ovarian cysts. Gray & Cameron’s review also noted that increased irritability, aggression, and masculine behavior had been observed in females following PZP-treatment.

PZP → Endocrine Disruptor → Elevated Testosterone → Masculinizing Effects

Recall that PZP has endocrine-disrupting effects that result in lowered estrogen. Per the observed masculine behavior of treated mares, PZP seems to have a testosterone-elevating effect too. A deficit of estrogen alone would not necessarily manifest in the masculinization of treated females, but an excess of testosterone would. So, it appears that PZP disrupts at least two hormones: estrogen — by substantially lowering it — and testosterone — by substantially elevating it. Adverse effect: Unnatural behavior.

PZP → Ovarian Cysts → Elevated Testosterone → Masculinizing Effects

As discussed above, PZP correlates with abnormal masculine behavior on the part of treated females, a side-effect likely due to elevated testosterone. But in addition to the endocrine-disruption caused by PZP, there could be a second way for testosterone levels to become elevated. Recall that PZP causes ovarian cysts. An Internet search on “ovarian cysts and testosterone” yielded results for polycystic ovary syndrome (PCOS) in women. Interestingly, one of the symptoms of PCOS is high testosterone levels. [12 and 22] The connection between ovarian cysts and elevated testosterone suggests that the ovarian cysts caused by PZP could — either alone or in combination with PZP’s endocrine-disruptor effects — lead to high testosterone levels in treated females, as evidenced by their masculinized behavior.

PZP Causes Additional Adverse Effects

Gray & Cameron’s review also disclosed that, when PZP was administered to the females of a herd, males lost body condition while the oft-claimed improvement in female body condition did not hold up. Further, mares remained sexually active beyond the normal breeding season and had more estrus events.

PZP Selects for Weak Immune Function

Gray & Cameron’s analysis raised the possibility of PZP selecting for immuno-compromised individuals. Here’s why. Because PZP stimulates the immune system, it ironically works “best” — sterilizes faster — in mares that have strong immune-function. Such mares respond to the anti-vaccine and produce quantities of PZP antibodies that destroy their ovaries. But, conversely, PZP may not work at all in mares whose immune-function is weak or depressed. Those mares fail to respond to PZP. They keep getting pregnant and producing foals who, like their dam, suffer from weak immune-function. So, the PZP pesticide works against the very horses that Nature has best equipped for survival against disease while favoring and selecting for the immuno-compromised. Thus, a herd being treated with PZP is undergoing selective breeding for weak immunity, which puts the population at risk for disease — and ultimately, for extinction.

PZP Confers Dubious “Benefit” of Increased Longevity

Gray & Cameron also cited a study that found that “… PZP treated feral horse mares lived longer, resulting in a new age class (>25 years) not present before treatment ….” Exceptionally-long life is an ironic effect of PZP treatments. PZP’s manufacturer actually boasts about it, as if the anomaly were a good thing. However, Gray & Cameron questioned the supposed benefit of mares living much longer than their normal life expectancy. Indeed, such mares take up scarce slots within size-restricted populations. The ultra-elderly mares continue to consume resources for many years, but they no longer contribute to the gene-pool. It is detrimental to a population’s genetic viability to carry significant numbers of sterile herd-members way-beyond their normal life-span.

Research on Wildlife Contraceptives Revealed Stillbirths and Auto-Immune Oophoritis from PZP

There was an even earlier, definitive meta-analysis on wildlife contraceptives. Nettles (1997) reviewed 75 studies available at that time on the subject. Among his findings regarding PZP-use across different species, including horses, were: Stillbirths; altered ovarian structure and cyclicity; interference with normal ovarian function; permanent ovarian damage; and some cases of irreversible sterility due to auto-immune oophoritis, which suggested that PZP can be selective against a certain genotype in a population. [10] Many of these findings were confirmed by Kaur & Prabha as well as by Gray & Cameron. Please keep in mind these key findings: Stillbirths, and auto-immune oophoritis.

Recent Stillbirths Correlated with PZP

There is recent evidence confirming Nettles’ finding of a correlation between PZP treatments and subsequent stillbirths. In June 2015, Karen Sussman, President of the International Society for the Protection of Mustangs and Burros, reported that 7 mares previously treated with PZP at ISPMB, when taken off PZP, were able to get pregnant. However, 6 of those 7 mares — that is, 86 percent — produced foals that were stillborn. All other ISPMB mares that had not been injected with PZP successfully birthed healthy foals. Thus, given that environmental and other conditions were identical, the only variable was PZP. The dead foals have been sent to a university pathology department for autopsy. [18]

Autoimmune Oophoritis Induced by PZP

Research by the Rose-Cihakova-Caturegli Laboratory at Johns Hopkins Pathology found: “Automimmune oophoritis can be induced by immunization with testis and ovarian antigen murine human zona pellucida 3 peptide (pZP3) in adjuvant.” [16] Here again, is causation of autoimmune disease by a ZP-type product. This finding confirms other research cited herein.

Autoimmune Oophoritis and Risk of Other Autoimmune Diseases

A study by Varras et al. disclosed that, in humans, autoimmune oophoritis carries the risk of the patient developing other autoimmune diseases. [23] The correlation between autoimmune oophoritis and subsequent other autoimmune disorders weighs against injecting fillies and mares with PZP repeatedly and en masse.

Prolonged Breeding Season, Unusually-late Parturition Dates with PZP

Nettles’ meta-analysis on PZP disclosed other adverse effects: A prolonged breeding season and unusually-late parturition dates. (Parturition is the formal term for “giving birth.”) These findings have recently been confirmed, as is discussed below.

Parturition-Season Extends to Nearly Year-Round When a Herd Is Treated with PZP

A longitudinal study (Ransom et al. 2013) of three herds currently being managed by PZP — Little Book Cliffs, McCullough Peaks, and Pryor Mountain — found that the the parturition season lasted 341 days. [15] Ransom et al.’s finding of a nearly year-round birthing season supports the earlier finding by Nettles. Thus, during its period of potential reversibility, PZP’s effects wear off unpredictably. Out-of-season births put the life of both the mare and the foal in jeopardy. Nature designed the equine birthing-season to occur in Spring, not year-round, and certainly not in the dead of Winter.

Prolonged Delay in Recovery of Fertility

The same longitudinal study by Ransom et al. found that, after suspension of PZP, there was a delay lasting 411.3 days (1.13 years) per each year-of-treatment before mares recovered their fertility. What this means is that it takes that long, on average, for the ovaries to heal, to clear out all those cysts, and to regain some degree of normal hormonal function.

The question is: How is the delay in recovery-of-fertility addressed by BLM management practices? Answer: BLM ignores it. For instance, BLM currently administers PZP to Pryor Mountain’s fillies and mares starting at age 1½ — whom BLM artfully describes in the Environmental Assessment as fillies “becoming two year olds” — through age four. Thus, these fillies and mares receive intentional treatments for four consecutive years before being allowed the privilege of reproductive potentiality. Per Ransom et al.’s study, the Pryor Mountain fillies and mares would be expected to need 1,645.2 days (4.51 years) to regain reproductive capacity. But BLM gives the Pryor Mountain mares only 5 years off PZP before they are put back on it again — for the rest of their life. Thus, these fillies and mares might have just a six-month window — at best — in which to conceive. Due to the unpredictable timing of PZP’s wearing off, for some mares that window of fertility will close before they get a chance to produce a foal. Those mares’ genetic contribution will be zero.

As if the above scenario were not bad enough, PZP’s manufacturer conceded that it could take up to eight years to recover fertility after just three consecutive PZP treatments. [13]

Ransom Advises Proceeding with Caution regarding PZP

The Ransom et al. study warned: “Humans are increasingly attempting to manage the planet’s wildlife and habitats with new tools that are often not fully understood. The transient nature of the immunocontraceptive PZP can manifest into extraordinary persistence of infertility with repeated vaccinations, and ultimately can alter birth phenology in horses. This persistence may be of benefit for managing overabundant wildlife, but also suggests caution for use in small refugia or breeding facilities maintained for repatriation of rare species.” [15]

Because BLM keeps over 70 percent of the herds at levels below minimum-viable population (MVP), most herds qualify as “small refugia.” Pryor Mountain WHR is a small, isolated refuge, and its wild horses carry genes with rare alleles.

Ransom’s Exclusion of Seven Mares Evidences PZP’s Non-Effect on Immunocompromised Mares

In the “Data Collection” methodology section of the Ransom et al. report, the authors advised: “We omitted data for one female from the Little Book Cliffs and six females from McCullough Peaks because they produced offspring in every treatment year and thus were never effectively contracepted.”

This fact is important because it evidences PZP’s lack-of-efficacy on immunocompromised fillies and mares. To review: Because PZP activates the immune system, mares with naturally-low or depressed immune function do not “respond” to the treatment. It’s as if they had been injected with saline — their immune system is so weak that it does not react to the PZP by producing antibodies. The good news is such mares’ ovaries are saved from PZP’s destructive effects. The bad news is that these mares continue to become pregnant year after year, producing foals that will also tend to inherit low immune-function. Over time, the herd will become populated with more such low-immune horses because those with strong immunity get sterilized. Thus, PZP selects for horses with low immune function, which is bad for a herd in the long term. Even a routine infection could spread quickly and wipe out a population of horses with weak immune-function. If the goal is to preserve a herd, the use of PZP constitutes a worst management-practice.

BLM Was Fully-Aware of the Ransom Study but Suppressed the Findings

In their report, the authors of the Ransom et al. study gave a shout-out to BLM “for administrative and technical support throughout this project.” Thus, BLM was fully aware of the multi-year study while it was in progress and even lent support to it administratively and technically. Yet, in the case of the Pryor Mountain herd, BLM omitted this important report as a reference for the 2015 Environmental Assessment, which proposed intensifying the PZP “prescription.” Thus, BLM pretended that there was no such report and unethicallly suppressed it. Consequently, the public could not comment knowledgeably and appropriately on the continued use of — let alone the accelerated application of — PZP.

Three PZP Injections Can Trigger Sterility in Mares, or Just One Shot in Fillies Before Puberty

Disturbingly, another recent study on PZP (Knight & Rubenstein, 2014) found that ” … three or more consecutive years of treatment or administration of the first dose before sexual maturity may have triggered infertility in some mares. [9]

These findings are particularly troubling. They suggest that, actually, only two consecutive PZP-treatments may be reversible. Except, that is, in the case of fillies who have not yet reached puberty — they could be sterilized by just one injection. Recall the Pryor Mountain fillies, whose PZP treatments begin when they are just 1½ years old. They may not have reached puberty when they are initially treated. [1] And as we shall see later in this report, that first shot of PZP may not be their first shot of PZP.

Researchers Again Express Concerns about the Abnormal Life-Spans of Sterilized Mares

Knight & Rubenstein warned: “Inducing sterility, while relieving the mares from the energetic costs of lactation and reducing the stress from harem switching, may have unintended consequences on population dynamics by increasing longevity and eliminating the mares’ ability to contribute genetically.”

Knight & Rubenstein’s concerns support those of Gray & Cameron, who also questioned the supposed benefit of sterile mares’ extended life-spans. The abnormal numbers of aged, sterile mares count for census-purposes; but their presence disadvantages the younger horses, who become tageted for removal in order for BLM to achieve arbitrary management levels. Further, such mares no longer belong to the viable gene-pool.

PZP’s Destructive Antibodies Are Transmitted via the Placenta and Mother’s Milk

It gets worse. Sacco et al. reported that, per radioimmunoassay, PZP antibodies are transferred from mother to young via the placenta and milk. The transferred antibodies cross-react with and bind to the zonae pellucidae of female offspring, as demonstrated by immunofluorescent techniques. [17] These findings were disclosed in 1981. PZP’s manufacturer must have known about this dangerous effect, and certainly BLM should have investigated on its own whether there was any risk to the unborn or the nursing foal. Yet, the manufacturer continued to insist that there was no danger to the foal, whether born or unborn. [7 and 13] And in fact, BLM regularly administers PZP to pregnant and lactating mares, who transfer the destructive antibodies to their fetus, via the placenta, and to their foal, via mother’s milk.

Recall again the Pryor Mountain fillies. If their dams were injected with PZP while pregnant or nursing, such fillies will already have PZP antibodies cross-reacted with and bound to their zonae. Therefore, when those same fillies are injected at age 1½, it will be their second treatment, or potentially even their third. In fact, they could already have been sterilized in utero or while nursing, the treatment having been received prior to puberty, about which Knight & Rubenstein warned.

PZP Weakens Herd-Immunity, Posing Risk of Stochastic Events Leading to Herd-Extinction

To be self-sustaining, a herd needs to possess good immunity to withstand random catastrophes — known as stochastic events — such as contagious infections. There was such an event recently in Kazakhstan, where 120,000 endangered Saiga antelope — half the world’s population — died off suddenly and inexplicably within a two-week period. Scientists think a common bacterial infection was the cause of this mass-mortality event, but are unsure why the antelope were unable to fight off the disease immunologically. [14]

Imagine if such a catastrophe were to befall the Pryor Mountain horses, whose herd-immunity is being eroded by PZP. Note that the Saiga deaths involved antelope-mothers and their calves. If Pryor Mountain’s few fertile mares and their foals perished all of a sudden, that would leave just stallions and sterile old mares. The herd would be composed of the living dead, reproductively speaking, its rare alleles extinguished. BLM is failing to proactively manage the Pryor Mountain herd with stochastic events taken into consideration. That is malfeasance. PZP is a tool of immunological destruction, not of proper management.

PZP Continues the Use of Roundups and Removals

If the promise of PZP were true — if PZP really did eliminate the need to remove “excess” wild horses from the range — removals would have ended long ago in the Pryor Mountain Wild Horse Range, where PZP has been in use for approximately two decades. Yet removals are scheduled there with regularity every three years, the latest one in 2015.

Risks to Humans Who Administer PZP Injections

For staff and volunteers who inject wild horses with PZP, EPA’s Pesticide Fact Sheet advises that Personal Protective Equipment requirements include long sleeved shirt and long pants, gloves and shoes plus socks to mitigate occupational exposure. EPA specifically warns that pregnant women must not be involved in handling or injecting ZonaStat-H, and that all women should be aware that accidental self-injection may cause infertility. [20]

However, EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to inform pregnant women why it is so important that they strictly avoid PZP — because PZP’s antibodies cross the placenta and cross-react with and bind to an unborn female child’s own little zonae pellucidae. The baby-girl could be “anti-vaccinated” with PZP and even sterilized before birth.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to warn lactating women to avoid PZP and why — because PZP’s destructive antibodies would be passed along to a nursing female child via mother’s milk. The baby-girl could be “anti-vaccinated” with PZP and possibly sterilized simply from nursing.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to warn all women of the risk of ovarian dystrophy, oophoritis, ovarian cysts, and elevated testosterone-levels — in addition to infertility and, potentially, sterility — from unintentional self-injection.

EPA’s Fact Sheet, the manufacturer’s training, and BLM’s operating procedures fail to emphasize the magnitude of the risk — the PZP-in-question is a horse-size dose.

But Is There a Mandate to Practice Scientific Integrity?

Yes. The Department of the Interior’s (DOI) Code of Scientific and Scholarly Conduct applies to all staff members as well as to contractors, partners, permittees, and volunteers. The Code states: “Scholarly information considered in Departmental decision making must be robust, of the highest quality, and the result of as rigorous scientific and scholarly processes as can be achieved. Most importantly, it must be trustworthy.” [19]

BLM has ignored and suppressed independent scientific findings about PZP’s adverse effects and unintended consequences. Instead, BLM continues to rely almost exclusively on the manufacturer’s claims — shown and known to be false — regarding PZP’s safety for use on horses and for handling by humans. BLM is thus non-compliant with the Policy and malfeasant in its responsibilities to protect staff, volunteers, and the wild horses under its jurisdiction. BLM is also misleading and disinforming Congress and the American public about the PZP pesticide.

The manufacturer of PZP — a partner to BLM — misrepresented the pesticide as safe for use on animals by humans. The manufacturer knew or should have known that the former hypothesis regarding PZP’s mode-of-action had been disproved, and that PZP has dangerous side effects, safety-issues, and unintended consequences. Yet he hid and denied that information and failed to warn about PZP’s adverse effects. The manufacturer cited his own research as if it were definitive, and aggressively criticized independent researchers whose findings did not fully support his claims. Indeed, he recently submitted an Op-ed to The Salt Lake Tribune wherein he belittled the research of fellow scientists whose studies on PZP yielded results somewhat different from his own. [8] His accusations were so unreasonable that the scientists felt it necessary to submit an Op-ed in response to defend the integrity and validity of their work. [11] The manufacturer also disparaged members of the public — one of whom was a member of the Pennsylvania Game Commission — who expressed concerns about PZP. He dismissively accused them of “an attempt to mislead,” of “hyperbole,” of “knowingly manipulating information,” of “attempts to frighten people,” and of indulging in an “anti-intellectual approach to debates.” [7] By these actions, the manufacturer violated the DOI’s Code of Scientific and Scholarly Conduct.

PZP Manufacturer Misled Trainees into Believing that PZP Was Safe

BLM staff and volunteers receive their training from PZP’s manufacturer in how to handle and administer the pesticide. BLM is remiss in delegating the training to the manufacturer without verifying the adequacy of the instruction and the truthfulness of it. Two comments recorded recently in the media suggest that PZP’s manufacturer misled not just the public-at-large but those who received training therefrom in how to administer PZP.

First, the manufacturer has been quoted as saying that PZP is “so safe it is boring.” [3] Independent research shows otherwise — that PZP is a powerful hormone disruptor that could sterilize a female with just one injection. If trainees believe that PZP is boringly safe, they will be less likely to protect themselves adequately from this dangerous pesticide. Indeed, many of the trainees are women and, therefore, particularly at risk. Likewise, wild-horse advocates are lulled into complacency, trusting that PZP is harmless to the Pryor Mountain horses and their rare genetic alleles. Of course, none of that is true.

Second, a PZP supporter, who self-identified as a recent completer of the PZP-darting training program conducted by the manufacturer, said in a comment posted to a news article: “I just received my FDA certification to handle and administer Native PZP. Would you be so kind to provide a link to the study you keep referencing? To my knowledge, and those teaching the Native PZP certification class, there are no side effects of the PZP produced by Dr. Kirkpatrick and his team, which is Native PZP.” [2] Key words: “no side effects.” It is disturbing that a person who was, no doubt, motivated by a desire to help the horses has been disinformed regarding PZP’s safety-hazards to humans as well as to horses.

BLM Fails to Maintain Proper Supervision of the PZP Volunteers

The issue of safety is not the only concern. As BLM has admitted, volunteers darted the wrong mares on Pryor Mountain. These errors evidence that BLM has failed to maintain supervisory control over the volunteer-inoculators, allowing them to conduct the PZP-darting by themselves. The mistakes further evidence that the volunteers do not understand what is expected of them. Who can say whether other procedures were not complied with either. The fact that mares were darted who were ineligible for PZP per the then-current protocol, but who would be eligible under the proposed-but-not-yet-promulgated new “prescription,” suggests that the volunteers may have concluded — from BLM’s open contempt for the Constitution and disrespect for the NEPA process — that was okay for them to start darting otherwise-ineligible mares right away. Not surprisingly, BLM blames the volunteers for these mistakes, but probably has not informed them that they are being made to take the rap for management’s shortcomings.

Conclusions

PZP is appropriately categorized as a pesticide by the EPA. PZP “works” by tricking the immune system into attacking and destroying the ovaries. PZP has many adverse effects as well as unintended consequences. PZP presents safety-hazards to humans who handle it. PZP is a dangerous pesticide whose use is antithetical to the spirit and intent of the Wild and Free-Roaming Horses and Burros Act. BLM’s continuing to use PZP while ignoring and suppressing the evidence of its harmful effects constitutes malfeasance.

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This report was completed by Marybeth Devlin on December 24, 2015. Copyright Marybeth Devlin and Protect Mustangs 2015.

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References:
1. EquiMed staff. (2010, March 13) Equine Reproductive Maturity in Mares and Stallions. Puberty in Equines. Retrieved from http://equimed.com/health-centers/reproductive-care/articles/equine-reproductive-maturity-in-mares-and-stallions

2. EWCS. (2015, November 10). Re: “Contraceptive could reduce taxpayer costs for wild horses.” Retrieved from http://wyomingpublicmedia.org/post/contraceptive-could-reduce-taxpayer-costs-wild-horses#comment-2352628323

3. Ferguson, Mike. (2015, June 4) “Police called as group protests wild horse contraceptives.” The Billings Gazette. Retrieved from http://billingsgazette.com/news/state-and-regional/montana/police-called-as-group-protests-wild-horse-contraceptives/article_81462303-e128-5ee8-a7ef-2c8b098450f6.html

4. Gray, M.E. and Cameron, E.Z. (2010) Does contraceptive treatment in wildlife result in side effects? A review of quantitative and anecdotal evidence. Reproduction 139, 45-55. Online publication date: 1-Jan-2010. Retrieved at http://www.reproduction-online.org/content/139/1/45.full

5. Kaur, Kiranjeet and Prabha, Vijay. (2014) “Immunocontraceptives: New Approaches to Fertility Control,” BioMed Research International, vol. 2014, Article ID 868196, 15 pages, 2014. doi:10.1155/2014/868196. Retrieved from http://downloads.hindawi.com/journals/bmri/2014/868196.pdf

6. Kirkpatrick, J. F., I. K. M. Liu, J. W. Turner, Jr., R. Naugle, and R. Keiper. 1992a. Long-term effects of porcine zonae pellucidae immunocontraception on ovarian function of feral horses (Equus caballus). J. Reprod. Fert. 94:437-444. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1317449

7. Kirkpatrick, Jay. 2007. “Response to PA [Pennsylvania] Game Commission.” Posted on PNC’s Wildlife Forever Home Page. Retrieved from http://www.pzpinfo.org/home.html

8. Kirkpatrick, Jay F. (2015, May 16). Op-ed: Wild-horse contraceptives are based on sound science. The Salt Lake Tribune. Retrieved from http://www.sltrib.com/opinion/2517266-155/op-ed-wild-horse-contraceptives-are-based-on?fullpage=1

9. Knight, Colleen M., Rubenstein, Daniel I. 2014. The Effects of Porcine Zona Pellucida Immunocontraception on Health and Behavior of Feral Horses (Equus caballus). Princeton University Thesis, Ecology and Evolutionary Biology. Retrieved from http://dataspace.princeton.edu/jspui/handle/88435/dsp01vt150j42p

10. Nettles, Victor F. (1997) Potential consequences and problems with wildlife contraceptives. Reproduction, Fertility and Development 9(1) 137 – 144. Retrieved from http://www.publish.csiro.au/paper/R96054.htm Accessed full pdf text via purchase of a copy from Csiro Publishing.

11. Nuñez, Cassandra, Jim Adelman and Dan Rubenstein. (2015, July 3). Op-ed: Wild horse contraception not without unintended consequences. The Salt Lake Tribune. Retrieved from http://www.sltrib.com/opinion/2653298-155/op-ed-wild-horse-contraception-not-without

12. PCOS Foundation. (2015) What Causes PCOS? Retrieved from http://www.pcosfoundation.org/what-is-pcos

13. PNC, Inc. (Pity Not Cruelty). PZP FAQs. (2006) “Frequently Asked Questions on Immunocontraception.” (Special thanks to Jay Kirkpatrick and Rick Naugle for additions and corrections). Retrieved from http://www.pzpinfo.org/pzp_faqs.html

14. Raab. Lauren. (2015, May 31) “120,000 endangered saiga antelopes die mysteriously in Kazakhstan.” Los Angeles Times. Retrieved from http://www.latimes.com/science/sciencenow/la-sci-sn-saiga-antelope-die-off-20150531-story.html

15. Ransom JI, Hobbs NT, Bruemmer J (2013) Contraception Can Lead to Trophic Asynchrony between Birth Pulse and Resources. PLoS ONE 8(1): e54972. doi:10.1371/journal.pone.0054972. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/23383018

16. Rose-Cihakova-Caturegli Laboratory. (n.d.) Autoimmune Oophoritis. Johns Hopkins Pathology. Retrieved from http://pathology.jhu.edu/department/RCCLab/Oophoritis.cfm

17. Sacco AG, Subramanian MG, Yurewicz EC. (1981) Passage of zona antibodies via placenta and milk following active immunization of female mice with porcine zonae pellucidae. J Reprod Immunol. 1981 Dec;3(6):313-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7328557

18. Sussman, Karen. (2015 June 6) “Suspicious Deaths with Use of Anti-Fertility Drugs.” International Society for the Protection of Mustangs and Burros. Retrieved from http://www.ispmb.org/BirthControlDeaths.html

19. United States Department of the Interior. Integrity of Scientific and Scholarly Activities Policy. Code of Conduct. Retrieved from https://www.doi.gov/scientificintegrity

20. United States Environmental Protection Agency. Office of Chemical Safety and Pollution Prevention. Pesticide Fact Sheet. Porcine Zona Pellucida (PZP). New Chemical. Nonfood Use. January 2012. Retrieved from
http://www3.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf

21. U.S. Department of Health and Human Services. National Institutes of Health. Endocrine Disruptors. Retrieved from http://www.niehs.nih.gov/health/topics/agents/endocrine/

22. U.S. National Library of Medicine. National Institutes of Health. Ovarian overproduction of androgens. Retrieved from https://www.nlm.nih.gov/medlineplus/ency/article/001165.htm

23. Varras M, Anastasiadis A, Panelos J, Balassi E, Demou A, Akrivis CH. (2013) Autoimmune oophoritis: Clinical presentation of an unusual clinical entity. OA Case Reports 2013 Jan 31;2(1):7. Retrieved from http://www.oapublishinglondon.com/article/369#

Pm PZP Darts

 

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Does PZP result in wild herds with lower immune systems and potential for die-offs?

PM Tule Elk Males FIghting by austlee

PZP is an immunocontraceptive and pesticide which causes an immune reaction to reject fertilization, while the females still come into estrus. Besides wrecking havoc on the immune system, injecting herds with PZP results in more fighting between males and many other behavior abnormalities.

Tule elk in Pt. Ryes National Seashore (Marin County, California) were part of a PZP (Porcine Zona Pellucida) experiment. Several years later there was a strange die-off.

Wildlife groups blamed park service management for leaving the elk fenced in during a drought–claiming that was the reason for the die-off.

Park service officials said the tule elk had water during the die-off.

“Some wildlife advocates have termed the situation a “die-off” and accuse the park service of allowing the elk to perish behind the fence that prevents them from finding enough food and water. Park service officials have a different view of what caused the population drop, and are hoping that new data will help address these concerns, especially as visitor interest peaks during the fall rutting season.” from: https://baynature.org/articles/on-the-fence/

Listen to Wildlife Ecologist Dave Press Discusses Tomales Point Elk and mention “there was water in the pond up there . . .” at 2:18.

 

It’s time to connect the dots and ask the obvious question: Did PZP lower the herds’ immune system and genetic diversity to the point of making them vulnerable to a die-off?

With suspect data regarding the long-term use of PZP on wild herds, more questions and answers are needed to prevent a similar die-off in America’s wild horses & burros.

With regards to wild horses, keep in mind what Marybeth Devlin wrote about PZP:

“PZP is a registered pesticide whose mechanism-of-action is to cause auto-immune disease. PZP tricks the immune system into producing antibodies that target and attack the ovaries. PZP’s antibodies cause the mare to suffer ovarian dystrophy, oophoritis (inflammation of the ovaries), ovarian cysts, destruction of oocytes in growing follicles, and depletion of resting follicles. Not surprisingly, estrogen levels drop markedly as the ovaries are slowly destroyed. But PZP’s adverse effects are not limited to the individual animal. As a recent study — which included the Little Book Cliffs, Colorado herd and the McCullough Peaks, Wyoming herd — found, PZP extends the birthing season to nearly year-round. Out-of-season births put the life of the foals and the mares at risk. Further, the same study disclosed that the pesticide causes a delay lasting 411.3 days (1.13 years) per each year-of-treatment before mares recover their fertility after suspension of PZP. However, some mares never recover — they are left permanently sterile, and quickly too. Indeed, yet another study found that sterility could occur in some mares from just three years of PZP injections or from just one treatment if the pesticide were given to a filly before she reached puberty. Because PZP messes with the immune system, it ironically works “best” — sterilizes faster — if the mare has a strong immune system. But, conversely, PZP may not work at all in mares whose immune function is weak or depressed. So, the pesticide discriminates against the very horses that Nature has best equipped for survival against disease while favoring and selecting for the immuno-compromised. Worse yet, tests performed via radioimmunoassay indicated that PZP antibodies are transferred from mother to young via the placenta and milk. The transferred antibodies cross-react with and bind to the zonae pellucidae of female offspring, as demonstrated by immunofluorescent techniques.”  [From: http://protectmustangs.org/?p=8529]

 

Pm PZP Darts

Links of interest™:

Immunocontraception (Wikipedia): https://en.wikipedia.org/wiki/Immunocontraception

“Whenever an immune response is provoked, there is some risk of autoimmunity. Therefore immunocontraception trials typically check for signs of autoimmune disease.[17] One concern with zona pellucida vaccination, in particular, is that in certain cases it appears to be correlated with ovarian pathogenesis.[2] However, ovarian disease has not been observed in every trial of zona pellucida vaccination, and when observed, has not always been irreversible.[18]”

 

Autoimmune disease (Wikipedia): https://en.wikipedia.org/wiki/Autoimmune_disease 

“Autoimmune diseases arise from an abnormal immune response of the body against substances and tissues normally present in the body (autoimmunity). . .”

 

ZonaStat-H is the EPA restricted-use pesticide–PZP–for wild horses and burros the registrant calls “pests”: http://www3.epa.gov/pesticides/chem_search/reg_actions/pending/fs_PC-176603_01-Jan-12.pdf

 

Tule elk: http://www.nps.gov/pore/learn/nature/tule_elk.htm

 

Tule elks at Pt. Reyes National Seashore (National Park Service): http://www.nps.gov/pore/getinvolved/supportyourpark/upload/volunteer_docent_info_tule_elk_elkmanagement_v5.0_1.pdf

 

Challenges face tule elk management in Point Reyes National Seashore  http://www.mercurynews.com/pets-animals/ci_28311296/challenges-face-tule-elk-management-point-reyes-national

“Earlier this year park service officials revealed that more than 250 tule elk died inside the fenced area over a two-year period, in part because pools that the herds rely on for water had gone dry. Meanwhile, ranchers are complaining about the free-range elk getting on their land and eating grass and drinking water intended for their dairy cattle and other agricultural operations.”

 

Paratuberculosis or Johne’s disease (Wikipedia): https://en.wikipedia.org/wiki/Paratuberculosis

 

Testing for Mycobacterium avium subsp. paratuberculosis infection in asymptomatic free-ranging tule elk from an infected herd.  http://www.ncbi.nlm.nih.gov/pubmed/12910759

“Forty-five adult tule elk (Cervus elaphus nannodes) in good physical condition were translocated from a population located at Point Reyes National Seashore, Marin County (California, USA), to a holding pen 6 mo prior to release in an unfenced region of the park. Because infection with Mycobacterium avium subsp. paratuberculosis (Mptb) had been reported in the source population, the translocated elk underwent extensive ante-mortem testing using three Johne’s disease assays: enzyme linked immunosorbent assay (ELISA); agar gel immunodiffusion assay (AGID), and fecal culture. Isolation of Mptb was made from fecal samples in six of 45 elk (13%). All AGID results were negative while ELISA results for 18 elk (40%) were considered elevated. Elevated ELISA results or Mptb isolation from fecal samples were obtained for 22 of 45 elk (49%); these elk were euthanized and necropsied. Mycobacterium avium subsp. paratuberculosis was isolated from tissue in 10 of 22 euthanized elk (45%); of these 10 cases of confirmed infection, eight had elevated ELISA results (80%) and four were fecal culture positive (40%). One of 10 cases had histopathologic lesions consistent with Mptb infection. Mycobacterium avium subsp. paratuberculosis was also isolated from tissue from one of eight fetuses sampled. The number of tule elk found to be infected was unexpected, both because of the continued overall health of the source herd and the normal clinical status of all study animals.”

 

Mycobacterium avium subspecies paratuberculosis and Mycobacterium avium subsp. avium infections in a tule elk (Cervus elaphus nannodes) herd. 2006. http://www.ncbi.nlm.nih.gov/pubmed/17255437 

Abstract
“Between 2 August and 22 September 2000, 37 hunter-killed tule elk (Cervus elaphus nannodes) were evaluated at the Grizzly Island Wildlife Area, California, USA, for evidence of paratuberculosis. Elk were examined post-mortem, and tissue and fecal samples were submitted for radiometric mycobacterial culture. Acid-fast isolates were identified by a multiplex polymerase chain reaction (PCR) that discriminates among members of the Mycobacterium avium complex (MAC). Histopathologic evaluations were completed, and animals were tested for antibodies using a Johne’s enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion. In addition, 104 fecal samples from tule elk remaining in the herd were collected from the ground and submitted for radiometric mycobacterial culture. No gross lesions were detected in any of the hunter-killed animals. Mycobacterium avium subsp. paratuberculosis (MAP) was cultured once from ileocecal tissue of one adult elk and was determined to be a strain (A18) found commonly in infected cattle. One or more isolates of Mycobacterium avium subsp. avium (MAA) were isolated from tissues of five additional adult elk. Gastrointestinal tract and lymph node tissues from 17 of the 37 elk (46%) examined had histopathologic lesions commonly seen with mycobacterial infection; however, acid-fast bacteria were not observed. All MAC infections were detected from adult elk (P = 0.023). In adult elk, a statistically significant association was found between MAA infection and ELISA sample-to-positive ratio (S/P) > or = 0.25 (P=0.021); four of five MAA culture-positive elk tested positive by ELISA. Sensitivity and specificity of ELISA S/P > or = 0.25 for detection of MAA in adult elk were 50% and 93%, respectively. No significant associations were found between MAC infection and sex or histopathologic lesions. Bacteriologic culture confirmed infection with MAP and MAA in this asymptomatic tule elk herd. The Johne’s ELISA was useful in signaling mycobacterial infection on a population basis but could not discriminate between MAA and MAP antibodies. The multiplex PCR was useful in discriminating among the closely related species belonging to MAC.
Between 2 August and 22 September 2000, 37 hunter-killed tule elk (Cervus elaphus nannodes) were evaluated at the Grizzly Island Wildlife Area, California, USA, for evidence of paratuberculosis. Elk were examined post-mortem, and tissue and fecal samples were submitted for radiometric mycobacterial culture. Acid-fast isolates were identified by a multiplex polymerase chain reaction (PCR) that discriminates among members of the Mycobacterium avium complex (MAC). Histopathologic evaluations were completed, and animals were tested for antibodies using a Johne’s enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion. In addition, 104 fecal samples from tule elk remaining in the herd were collected from the ground and submitted for radiometric mycobacterial culture. No gross lesions were detected in any of the hunter-killed animals. Mycobacterium avium subsp. paratuberculosis (MAP) was cultured once from ileocecal tissue of one adult elk and was determined to be a strain (A18) found commonly in infected cattle. One or more isolates of Mycobacterium avium subsp. avium (MAA) were isolated from tissues of five additional adult elk. Gastrointestinal tract and lymph node tissues from 17 of the 37 elk (46%) examined had histopathologic lesions commonly seen with mycobacterial infection; however, acid-fast bacteria were not observed. All MAC infections were detected from adult elk (P = 0.023). In adult elk, a statistically significant association was found between MAA infection and ELISA sample-to-positive ratio (S/P) > or = 0.25 (P=0.021); four of five MAA culture-positive elk tested positive by ELISA. Sensitivity and specificity of ELISA S/P > or = 0.25 for detection of MAA in adult elk were 50% and 93%, respectively. No significant associations were found between MAC infection and sex or histopathologic lesions. Bacteriologic culture confirmed infection with MAP and MAA in this asymptomatic tule elk herd. The Johne’s ELISA was useful in signaling mycobacterial infection on a population basis but could not discriminate between MAA and MAP antibodies. The multiplex PCR was useful in discriminating among the closely related species belonging to MAC.”

 

Epizootic of paratuberculosis in farmed elk http://www.johnes.org/handouts/files/Elk_outbreak.pdf

 

TESTING FOR MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS INFECTION IN ASYMPTOMATIC FREE-RANGING TULE ELK FROM AN INFECTED HERD (Journal of Wildlife Diseases, : http://www.bioone.org/doi/pdf/10.7589/0090-3558-39.2.323

 

Immuno-Contraception Research for Managing Tule Elk Population – Phase I Scheduled to Begin on August 6, 1997 http://www.nps.gov/pore/learn/news/newsreleases_19970805_elkimmunocontraception97.htm

“. . . Funding for tule elk projects has come from a variety of sources. To date, monetary support and in-kind services for the tule elk project has been received from the Rocky Mountain Elk Foundation, Point Reyes National Seashore Association, Committee for the Preservation of Tule Elk, California Department of Fish and Game, The Humane Society of the United States (HSUS), University of California at Davis, the National Park Service Natural Resource Preservation Program and In Defense of Animals.” [Evidently Suzanne Roy, currently the Director of the American Wild Horse Preservation Campaign–who pushes PZP based management–was working for IDA at the time.]

 

Immuno-Contraception Research for Managing Tule Elk Population – Phase II Scheduled to Begin on June 15, 1998  http://www.nps.gov/pore/learn/news/newsreleases_19980615_elkimmunocontraception98.htm

“. . . During the second phase of the contraceptive research project, the first vaccine will be administered by direct syringe injection. To administer the injection, 30 elk will be captured from a helicopter and hobbled by ground crews. Scientists will gather data on the individual elk and place a radio collar on each of the elk. The collar will allow scientists to follow the individual elk to determine the effectiveness of the contraceptive. After several weeks, a booster shot will be remotely administered, from ranges of 30 to 150 feet, by means of self-injecting darts. The darts are brightly colored and easily retrieved. A single annual booster inoculation will be administered to continue contraceptive effects for successive breeding seasons.”

 

Use of porcine zona pellucida (PZP) vaccine as a contraceptive agent in free-ranging tule elk (Cervus elaphus nannodes). published 2002: http://www.ncbi.nlm.nih.gov/pubmed/12220156 

Abstract (note only a 5 year study. Why aren’t they studying the truly long-term effects?)
The potential for the application of porcine zona pellucida (PZP) immunocontraception in wildlife population management has been tested over a 15 year period and promises to provide a useful wildlife management tool. These studies have provided evidence indicating that the use of PZP immunocontraception in wildlife: (i) is effective at both the physiological and population level (Liu et al., 1989; Kirkpatrick et al., 1996; Turner et al., this supplement); (ii) is deliverable by remote means (Kirkpatrick et al., 1990; Shideler, 2000); (iii) is safe in pregnant animals (Kirkpatrick and Turner, this supplement); (iv) is reversible (Kirkpatrick et al., 1991; Kirkpatrick and Turner, this supplement); (v) results in no long-term debilitating health problems (Kirkpatrick et al., 1995; Turner and Kirkpatrick, this supplement); (vi) has no implications for passage through the food chain (Harlow and Lane, 1988); and (vii) is reasonably inexpensive (J. F. Kirkpatrick, personal communication). This report presents the results of a 5 year study in tule elk (Cervus elaphus nannodes), 3 years of which were on the application of PZP immunocontraception to an expanding elk population living in a wilderness area of Point Reyes National Seashore in Marin County, CA…”

 

Copyright Protect Mustangs.org 2016





Feds want to use 11 million tax dollars to experiment on American Wild Horses and Burros

Note from Protect Mustangs: If you don’t like this then: 1.) Go see your congressional representative this week and ask them to intervene to stop these horrible experiments on America’s wild horses who are being managed to extinction. 2.) Sign and share this petition and email it to everyone you know: https://www.change.org/p/defund-and-stop-the-wild-horse-burro-roundups Groups like The Cloud Foundation and the coalition led by The American Wild Horse Preservation Campaign seem to be misleading the public because they have chosen pushing PZP (controlled by The Humane Society of the United States) over championing wild horse freedom on public land. They slip appeals for PZP in the bottom of their online petitions hoping the public won’t notice what they are signing. That was the beginning of this slippery slope towards experimentation and extinction. Why? Follow the money, fear mongering and the seduction to campaign for drugging wild horses and burros with a risky pesticide made from slaughterhouse pig ovaries to block fertility. . . 3.) It’s time to join Protect Mustangs to protect our national treasures. Go to www.ProtectMustangs.org to sign up. 4.) You can donate to the Wild Horse Legal Fund also. The crowd funding link is here: https://www.gofundme.com/MustangLaw2016 or donate by www.PayPal.com to Contact@ProtectMustangs.org and please mark your donation is for the “Legal Fund”. Thank you for taking action today! Together we can turn this around.





The Bureau of Land Management (BLM) wants to use American tax dollars in several cruel experiments to develop methods of wild horse and burro population control–despite the fact that there is no overpopulation of wild horses or burros. The BLM anticipates the total cost of the experiments to be $11 million over 5 years.The research is being conducted by university scientists as well as scientists from the U.S. Geological Survey.

Research with Universities results in experimenting on wild horses and burros

In its 2013 report to the BLM, the National Academy of Sciences (NAS) found that no highly effective, easily delivered and affordable fertility-control methods were currently available for use on wild horses and burros. The most promising birth control, PZP, made from slaughterhouse pig ovaries, is limited in the duration of its effectiveness (1-2 years). At the same time, after multiple applications or if applied to young fillies it permanently sterilizes native wild horses.

The BLM released a solicitation for experimentation to develop new or improve existing population growth suppression methods for wild horses. (http://www.blm.gov/wo/st/en/info/newsroom/2015/july/nr_07_07_2015.html)  The following seven research projects were reviewed and recommended by an NAS panel of experts and are consistent with recommendations made to the BLM by its Wild Horse and Burro Advisory Board who is biased against wild horses and prefers livestock use public land for cheap grazing.

Wild Horse & Burro Advisory Board Meeting in 2013

 

© EquineClinic.comn shared for educational purposes

© EquineClinic.comn shared for educational purposes

1. Evaluation of minimally invasive methods of contraception in wild horse and burro mares: tubal ligation and hysteroscopically-guided oviduct papilla laser ablation. This was pushed by pro-slaughter advocates who want the horses free of fertility control drugs so they can go to slaughter eventually.

Recipient: Oregon State University
Summary: A one-year experiment that will aim to develop a minimally invasive surgical sterilization method for wild horse mares that requires no incisions.
Details: In an effort to develop minimally invasive, low-risk techniques for contraception and population control in female wild horses and burros, the experiment will evaluate two procedures, tubal ligation and hysteroscopically-guided laser ablation of the oviduct papilla in standing sedated females. For tubal ligation, the research team hypothesizes that a flexible endoscope inserted through a small incision in the vaginal vault will allow visualization of each oviduct in mares. Use of a diode laser or cautery instrument will allow effective fulguration followed by bloodless sectioning of the oviduct. This procedure should allow successful sterilization of up to 100% of female wild horses and burros gathered in any particular location as a single event. For the hysteroscopic procedure, the recipients expect to endoscopically visualize each oviduct papilla in standing, sedated, non-pregnant mares. A diode laser will be used to seal the opening between the oviduct and each uterine horn, thus preventing subsequent fertilization. The proposed procedures do not involve major surgery, are expected to have minimal complications while approaching 100% effectiveness, and when applied, are expected to result in a static to decreasing population level. Additionally, tubal ligation is a technique commonly performed in humans. The development of an acceptable sterilization technique will help control the population levels of wild horses and burros.


2. Tubo-ovarian ligation via colpotomy as a method for sterilization in mares

Recipient: University of Kentucky
Summary: A two-year experiment to develop different surgical approaches for tubal ligation in mares.
Details: The overall goal of this experiment is to develop methodology for the safe, economical and effective sterilizationof mares via colpotomy (vaginal incision) to achieve: 1) ovarian necrosis / atrophy via application of a ligature to the ovarian pedicle and 2) simultaneous sterilization via tubal ligation (i.e., tubo-ovarian ligation). The project will help determine the effectiveness of a custom-designed instrument for placement of a polyamide (nylon) cable tie around the ovarian pedicle and oviduct of mares via colpotomy for tubo-ovarian ligation. The procedure, conducted in the standing animal under sedation and local anesthesia, is expected to induce permanent sterilization of treated mares. The researchers will assess any post-operative complications of the procedure in mares and the effects on the health of mares to determine long-term effects on the reproductive tract, the overall health of mares and the fertility of mares undergoing the procedure, and the feasibility of these procedures in pregnant mares.

PM Sick Filly PVC March 25 2014
3. Functional assessment of ovariectomy (spaying) via colpotomy of wild mares as an acceptable method of contraception and wild horse population control

Recipient: Oregon State University
Summary: A six-month experiment that will determine whether an existing accepted surgical sterilization procedure commonly used for domestic mares can be safely conducted on wild horses.
Details: This experiment proposes to conduct a large-scope investigation of the safety and practicality of spaying mares as a tool for wild horse population control. Specifically, the researchers will help determine whether ovariectomy via vaginal colpotomy can be safely and effectively performed on wild mares that have been selected for non-breeding status. Non-breeding horses could then be returned to the range to live out their natural lives without individually contributing to population growth. The proposed research effort is based on recent pilot studies that have suggested the potential for surgery-related health complications from ovariectomy in adult female horses is low (near 1%). When evaluating options for field techniques, spaying (ovariectomizing) mares as a population control method is not recommended unless it can be performed in a safe, practical, and effective manner. The results of this study will provide standardized, baseline outcomes for this surgical procedure which can be directly compared to other less invasive procedures being conducted and evaluated by the same research team.

PM WC11 Lucky 11 Map

Map of Western United States showing 12 current field research/pilot projects.

4. Re-immunization of Free-Ranging Horses with GonaCon Immunological Vaccine: Effects on Reproduction, Safety, and Population Performance

Recipient: Colorado State University
Summary: A two-year experiment will focus on further study of Gonocon, an approved and labeled contraceptive vaccine for equids.

PM PZP Injection
Details: This experiment will focus on the effectiveness of GonaCon as an immunological vaccine, with five objectives: 1) to begin to determine the optimum and most effective re-vaccination schedule with GonaCon vaccine for suppressing reproductive rates in free-ranging horses, the duration of effectiveness, and the return to fertility following treatment; 2) to determine the safety and physiological side-effects (if any) in feral horses following re-vaccination with GonaCon including visual assessment of general health, body condition, injection site reactions, effects on current pregnancy, and neonatal health and survival; 3) to determine the effects of GonaCon vaccination on the behavioral side-effects (if any) in free-ranging horses including quantitative assessment of the effects on daily activity patterns and social interactions; 4) to develop and test a safe and effective dart configuration and injection system for remotely administering GonaCon vaccine to free-ranging horses by means of a syringe dart; and 5) to develop a Bayesian model to forecast the consequences of different GonaCon vaccine treatments on feral horse population dynamics at THRO. [Teddy Roosevelt National Park].


5. The Effect of Immunization against Oocyte Specific Growth Factors in Mares

Recipient: Colorado State University
Summary: A two-year experiment to develop a new, permanent contraceptive vaccine for wild horse mares.
Details: This experiment will focus on vaccination against two key proteins in wild horse and burro females, either alone or in combination, which may result in permanent sterility through premature oocyte depletion. The depletion of oocytes may occur by simply causing them all to become atretic prematurely and/or accelerating the process so that after a single season the mares and jennies have depleted their oocyte reserves. To test this hypothesis, the researchers will vaccinate mares against the proteins and track their sexual behavior, follicular growth, hormonal profile and ultimately total oocyte count over a two-year period. The long-term goal is to develop a vaccine that can cause permanent sterility after a single dose.

PM Burros Wild 2 © Carl Mrozek

Cruel way to drag foal by pulling bailing twine around their neck (Photo © Bo Rodriguez)

Cruel way to drag foal by pulling bailing twine around their neck (Photo © Bo Rodriguez)

6. Electrospun delivery to enhance the effectiveness of immunocontraception strategies in equids

Recipient: Ohio State University
Summary: A four-year experiment that will attempt to develop a new delivery vehicle for porcine zona pellucida (PZP) – a temporary contraceptive currently used in some wild horse herds – that would increase the duration of the vaccine’s effectiveness.
Details: To reduce population on public lands, horse immunocontraception has largely focused on the use of PZP in free-roaming wild populations. The vaccine appears to act by stimulating anti-PZP antibodies that bind to the surface of the ovulated egg, preventing sperm attachment. While performance has been satisfactory, recent results have been associated with contraceptive efficiencies that are considerably less than 100%. The basis for this is unknown but is believed to be in part caused by delivery methods that require substantial heating during polymer vehicle fabrication, expose PZP to enzymatic fluids prior to entry into the bloodstream and allow gradual – not burst – release. Gradual release can potentially desensitize the immune system to the presence of PZP, resulting in inferior production of anti-PZP antibodies. Thus, an ideal delivery method would allow release of PZP in “bursts” at pre-determined intervals to assure constant immune stimulation. This project will seek to develop an electrospun technology that can allow long-term, ‘burst’ delivery of porcine zona pellucida (PZP) vaccines to the intramuscular environment of horses and burros to result in prolonged suppression of reproduction. For large-scale application, free roaming horses could be gathered in the field and processed through stock chutes for aging, at which time the implants will be inserted by trocar. The experiment will also carry out parallel in vitro and in vivo experiments to examine the potential of electrospun vehicles as immunocontraceptive carriers. An electrospun “universal delivery vehicle” will be developed to provide sustained release of effective levels of porcine zona pellucida (PZP) for immunocontraception over periods of at least three years. By careful design, fabrication and testing of two different electrospun designs, the researchers will create a comprehensive evaluation of this novel method of delivery.

Pm PZP Darts
7. The use of membrane disrupting peptide / peptoid LHRH conjugates to control wild horse and burro populations

Recipient: Louisiana State University
Summary: A three-year experiment for the development of an injectable agent that would inactivate hormones and decrease female and male gonad viability.
Details: The experiment is a multidisciplinary effort aimed at developing novel drugs to control wild horse and burro populations. Several types of drugs consisting of conjugates of membrane disrupting peptides (such as Phor 21) with luteinizing hormone releasing hormone (LHRH) currently exist. These drugs (such as LHRH-Phor 21 conjugate) effectively target, bind to and destroy prostate, testicular, breast and ovarian cancer cells, as well as testicular and ovarian cells that control reproduction. LHRH targets the cell and delivers Phor 21 to the cancer cell or the reproductive cell in the testes or ovary and destroys it. Preliminary experiments suggest that administration of this drug by a slow-release delivery system will destroy the cells that control spermatogenesis in the male and follicle growth, oocyte development, ovulation and cyclicity in the female. Preliminaryresults also show that LHRH-Phor 21 targets and destroys gonadotropic cells in the pituitary gland. This indicates that cessation of reproductive activity is the result of both central control at the level of the pituitary gland and on receptor binding cells in both male and female gonads. The experiment will also assess the effect the drugs have on pregnant mares, both in early gestation and late gestation.

PM PZP Syringe Yearling Meme

Additional details about these experiments can be found in the following documents:

Detailed Summary of University-led Experiments for Fertility Control Tools for Wild Horses
Review of Proposals to the BLM on Wild Horse and Burro Sterilization or Contraception: A Letter Report
Research with the U.S. Geological Survey

Through its partnership with U.S. Geological Survey (USGS), the BLM is undertaking important research aimed at delivering better methods and tools for managing wild horse and burro herds on public lands. These projects build upon on-going cooperation between the BLM and USGS that is implementing new methods to estimate wild horse and burro population size.

There are nine USGS experiments that have been approved or are on-going:
Collaring & radio marking (1 year): The aim is to develop safe GPS collars for tracking animals to determine habitat selection, movement ecology, population estimation, behavior, etc. GPS tracking might also help locating animals for contraceptive treatments.
Fecal DNA (genetics/population survey) (1.5 years): The experiment involves the collection and analysis of fecal DNA as a noninvasive method to determine genetic diversity and estimate population size.
Carrying capacity modeling (1 year): This experiment’s aim is to develop a coarse model to evaluate changes in animal carrying capacity in response to changes in vegetation production. The resulting model may help BLM to adapt plans in response to climatic change.

PM PZP Syringe FB
Mare Contraception -SpayVac Pen Trial II (5 years): This experiment will help determine the efficacy of alternative SpayVac contraceptive vaccine formulations that are potentially longer acting than conventional PZP vaccines.
Evaluating Behavior of Spayed Free-Roaming Mares (4 years): The experiment will determine the effects of spaying on behavior, interactions, and movement of spayed mares among a breeding herd. The study will also determine the population level effect on herd growth.
Evaluating Behavior of Geldings among a Breeding Herd (4 years): This experiment will determine any effects of gelding on behavior, movement, interactions and changes in habitat selection.
Two Sentinel Horse Herd Management Area (HMA) Demography Studies (2 studies, each of 5 years): These experiments will provide demographic data sets for use in new population models and serve as control HMAs for gelding and spayed mare field studies.
Burro Sentinel HMA Demography Study (5 years): The experiment will involve collecting data on the survival, fertility, fecundity, recruitment, movements, range use, habitat selection and social behavior of wild burros. These data will be used in population modeling.
The BLM has requested or is reviewing proposals for the following projects with USGS:
Evaluate the Use of a Silastic O-Ring Intrauterine Device (IUD) in Mares (4 years): This experiment will determine any effects on mare health resulting from the long-term presence of the silastic O-ring IUD. This IUD has effectively prevented pregnancy in domestic mares during one breeding season.
Burro Population Survey Method Development (2.5 years): This experiment will test two new population survey methods for wild burros. The existing simultaneous double-observer method, when applied to burros, tends to lead to underestimates of true burro population size.
WinEquus II – Population Model with Cost/Benefit Outputs (1.5 years): This experiment will develop a model that compares population modeling outcomes and projects the costs, benefits and expected population growth resulting from management actions that involve PZP, removals, spaying, gelding and other population growth suppression tools.
Testing Efficacy of Contraceptives for Female Burros (3-4 years): Contraceptive vaccines have yet to be used on wild burros due to limited research and unknown effects. This study will examine the efficacy of various existing vaccines.

PM Hazard Foter Public domain Marked Sterilize

© Protect Mustangs, 2016


Conversation about fertility control with Brett Hass, retired biologist previously with NSA (part 1)

Don’t let pesticides like Gonacon™, PZP or SpayVac® manage native wild horses to extinction. The Spin Dr.’s and some ignorant horse advocates are pushing fertility control on underpopulated wild horses. Know the truth and expose those spewing the spin.

PM Gonacon Pesticide Fact Sheet

Read the entire Gonacon™ Pesticide Fact Sheet

Interview

John Cox: Brett, you’re a retired biologist, previously with the NSA for how many years?

Brett Hass: 46 long years John. We met in Vietnam, when we a lot younger my friend (smiles). We were doing wildlife and vegetation studies in your AO (area of operations). That was my first assignment with the NSA, as a matter of fact.

John Cox: Let’s cut through all of that. What do you think of Gonacon™ and some of the other fertility controls BLM is using?

Brett Hass: As usual, and as government agencies do and BLM and DOI are extremely guilty, they pretend that science guides its wild horse and burro management strategies. So the agencies involved go forth and resolve issues, supposedly, with experimental drugs–in this case GonaCon™. The question is, in reality, does it resolve anything at all, or is it simply to further experimentations with this drug and the wild horses the most expendable of situations currently?

The problem is very obvious, with the first-time use of an experimental drug, they act like children with a new toy–but this toy is extremely dangerous, to not only the horses and actually lead them to extinction, but the environmental consequences are apparently neglected entirely? (shakes his head negatively). . .

But let’s get real, the very absence of science contradicts any time of sound reasoning for its use whatsoever. So once again we have a government agency, using a Nazi-Type experimental fertility drug on horses and other wildlife–without knowing, or even acknowledging for that matter, what the long-term consequences are–or in perception–the problems that will arise in its actual use.

There is no resolution, as I reviewed the population situation and see first hand there is no over-population if wild horses just within the BLM numbers alone; but livestock, that is a completely different matter, in reality.

John Cox: In your appraisal of the information you’ve read, would you, as a tenured Wildlife Biologist for over 50 years, use this drug?

Brett Hass: Absolutely not! There is no pertinent reasoning to use it right now and especially on wild horses or much of anything else for that matter. Our wildlife and environment is simply too important to be so frivolous with such activity, especially an unknown situation, as fertility drugs used in our natural environment. But as you say, ignorance is quite something, and our government seems to portray ignorance quite well, and frankly the only situation they seem to be competent to accomplish.

Much more on this discussion with Brett Hass later . . .

574px-Blm.svg

Below is the original PZP Pesticide Fact Sheet before HSUS seems to have lobbied the EPA to make changes to the chemical class. It’s still only approved as a pesticide to manage pests. PZP is made from slaughterhouse pig ovaries mixed with modified freund’s complete adjuvant.

PM PZP Test mares

(American wild horses used in fertility control experiments)

© John Cox, printed with permission

John Cox is a Vietnam Vet, living in the mountains of the Pacific Northwest who writes about the environment. He’s passionate about saving America’s wild horse herds and wolves. 

Read John Cox’s blog at: https://prophoto7journal.wordpress.com

Have you read about the Gonacon™ Experiment on the Water Canyon herd and the UNLUCKY 11 orphans? It all started as a PZP proposal and went down the slippery slope. . .  Read about it here: http://protectmustangs.org/?p=8488